Functional assignment to positively selected sites in the core type III effector RipG7 from Ralstonia solanacearum

Wang, Keke; Remigi, Philippe; Anisimova, Maria; Lonjon, Fabien; Kars, Ilona; Kajava, Andrey; Li, Chien-Hui; Cheng, Chiu-Ping; Vailleau, Fabienne; Genin, Stéphane; Peeters, Nemo

doi:10.1111/mpp.12302

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Functional assignment to positively selected sites in the core type III effector RipG7 from Ralstonia solanacearum
Authors:	Wang, Keke Remigi, Philippe Anisimova, Maria Lonjon, Fabien Kars, Ilona Kajava, Andrey Li, Chien-Hui Cheng, Chiu-Ping Vailleau, Fabienne Genin, Stéphane Peeters, Nemo
DOI:	10.1111/mpp.12302
Published in:	Molecular Plant Pathology
Volume(Issue):	17
Issue:	4
Page(s):	553
Pages to:	564
Issue Date:	2016
Publisher / Ed. Institution:	Wiley
ISSN:	1464-6722 1364-3703
Language:	English
Subjects:	LRR; Medicago truncatula; Ralstonia solanacearum; positive selection; Type III effector; Virulence function; Amino acid sequence; Bacterial protein; Genetic complementation test; Medicago truncatula; Molecular model; Site-directed mutagenesis; Mutation; Phylogeny; Plant disease; Ralstonia solanacearum; Amino acid sequence homology; Virulence; Bacterial secretion system; Genetic selection
Subject (DDC):	570: Biology 580: Plants (Botany)
Abstract:	The soil-borne pathogen Ralstonia solanacearum causes bacterial wilt in a broad range of plants. The main virulence determinants of R. solanacearum are the type III secretion system (T3SS) and its associated type III effectors (T3Es), translocated into the host cells. Of the conserved T3Es among R. solanacearum strains, the Fbox protein RipG7 is required for R. solanacearum pathogenesis on Medicago truncatula. In this work, we describe the natural ripG7 variability existing in the R. solanacearum species complex. We show that eight representative ripG7 orthologues have different contributions to pathogenicity on M. truncatula: only ripG7 from Asian or African strains can complement the absence of ripG7 in GMI1000 (Asian reference strain). Nonetheless, RipG7 proteins from American and Indonesian strains can still interact with M. truncatula SKP1-like/MSKa protein, essential for the function of RipG7 in virulence. This indicates that the absence of complementation is most likely a result of the variability in the leucine-rich repeat (LRR) domain of RipG7. We identified 11 sites under positive selection in the LRR domains of RipG7. By studying the functional impact of these 11 sites, we show the contribution of five positively selected sites for the function of RipG7CMR15 in M. truncatula colonization. This work reveals the genetic and functional variation of the essential core T3E RipG7 from R. solanacearum. This analysis is the first of its kind on an essential disease-controlling T3E, and sheds light on the co-evolutionary arms race between the bacterium and its hosts.
URI:	https://digitalcollection.zhaw.ch/handle/11475/8259
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Computational Life Sciences (ICLS)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Wang, K., Remigi, P., Anisimova, M., Lonjon, F., Kars, I., Kajava, A., Li, C.-H., Cheng, C.-P., Vailleau, F., Genin, S., & Peeters, N. (2016). Functional assignment to positively selected sites in the core type III effector RipG7 from Ralstonia solanacearum. Molecular Plant Pathology, 17(4), 553–564. https://doi.org/10.1111/mpp.12302

Wang, K. et al. (2016) ‘Functional assignment to positively selected sites in the core type III effector RipG7 from Ralstonia solanacearum’, Molecular Plant Pathology, 17(4), pp. 553–564. Available at: https://doi.org/10.1111/mpp.12302.

K. Wang et al., “Functional assignment to positively selected sites in the core type III effector RipG7 from Ralstonia solanacearum,” Molecular Plant Pathology, vol. 17, no. 4, pp. 553–564, 2016, doi: 10.1111/mpp.12302.

WANG, Keke, Philippe REMIGI, Maria ANISIMOVA, Fabien LONJON, Ilona KARS, Andrey KAJAVA, Chien-Hui LI, Chiu-Ping CHENG, Fabienne VAILLEAU, Stéphane GENIN und Nemo PEETERS, 2016. Functional assignment to positively selected sites in the core type III effector RipG7 from Ralstonia solanacearum. Molecular Plant Pathology. 2016. Bd. 17, Nr. 4, S. 553–564. DOI 10.1111/mpp.12302

Wang, Keke, Philippe Remigi, Maria Anisimova, Fabien Lonjon, Ilona Kars, Andrey Kajava, Chien-Hui Li, et al. 2016. “Functional Assignment to Positively Selected Sites in the Core Type III Effector RipG7 from Ralstonia Solanacearum.” Molecular Plant Pathology 17 (4): 553–64. https://doi.org/10.1111/mpp.12302.

Wang, Keke, et al. “Functional Assignment to Positively Selected Sites in the Core Type III Effector RipG7 from Ralstonia Solanacearum.” Molecular Plant Pathology, vol. 17, no. 4, 2016, pp. 553–64, https://doi.org/10.1111/mpp.12302.