| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology |
| Autor/-in: | Schönborn, Andreas Schmid, Pascal Bräm, Sarah Reifferscheid, Georg Ohlig, Marina Buchinger, Sebastian |
| DOI: | 10.1016/j.chroma.2017.11.009 |
| Erschienen in: | Journal of Chromatography A |
| Band(Heft): | 1530 |
| Seite(n): | 185 |
| Seiten bis: | 191 |
| Erscheinungsdatum: | 29-Dez-2017 |
| Verlag / Hrsg. Institution: | Elsevier |
| ISSN: | 0021-9673 1873-3778 |
| Sprache: | Englisch |
| Schlagwörter: | Planar-YES; Estrogenic compound; HPTLC; Hyphenation; Spray-on procedure; Sensitivity |
| Fachgebiet (DDC): | 572: Biochemie |
| Zusammenfassung: | The planar yeast estrogen screen (p-YES) can serve as a highly valuable and sensitive screening tool for the detection of estrogenic compounds in various sample matrices such as water and wastewater, personal care products and foodstuff. The method combines the separation of sample constituents by thin layer chromatography with the direct detection of estrogenic compounds on the surface of the HPTLC-plate. The previous protocol using the immersion of a normal phase silica HPTLC-plate in a cell suspension for bio-autography resulted in blurred signals due to the accelerated diffusion of compounds on the wet surface of the HPTLC-plate. Here, the application of the yeast cells by spraying on the surface of the HPTLC-plate is described as an alternative approach. The presented method for the hyphenation of normal phase thin layer chromatography with a yeast estrogen screen results in much sharper signals compared to reports in previous publications. Satisfying results were achieved using cultures with cell densities of 1000 FAU. Due to the reduced signal broadening, lower limits of quantification for estrogenic compounds were achieved (Estrone (E1) = 2 pg/zone, 17β-estradiol (E2) = 0.5 pg/zone, 17α-ethinylestradiol (EE2) = 0.5 pg/zone and Estriol (E3) = 20 pg/zone). As demonstrated, it is possible to characterize profiles of estrogenic activity of wastewater samples with high quality and reproducibility. The improved sensitivity opens the stage for applications using native samples from waste- or even surface water directly applied on HPTLC-plates without the need for prior sample treatment by e.g. solid phase extraction. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/7317 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Umwelt und Natürliche Ressourcen (IUNR) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Schönborn, A., Schmid, P., Bräm, S., Reifferscheid, G., Ohlig, M., & Buchinger, S. (2017). Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology. Journal of Chromatography A, 1530, 185–191. https://doi.org/10.1016/j.chroma.2017.11.009
Schönborn, A. et al. (2017) ‘Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology’, Journal of Chromatography A, 1530, pp. 185–191. Available at: https://doi.org/10.1016/j.chroma.2017.11.009.
A. Schönborn, P. Schmid, S. Bräm, G. Reifferscheid, M. Ohlig, and S. Buchinger, “Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology,” Journal of Chromatography A, vol. 1530, pp. 185–191, Dec. 2017, doi: 10.1016/j.chroma.2017.11.009.
SCHÖNBORN, Andreas, Pascal SCHMID, Sarah BRÄM, Georg REIFFERSCHEID, Marina OHLIG und Sebastian BUCHINGER, 2017. Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology. Journal of Chromatography A. 29 Dezember 2017. Bd. 1530, S. 185–191. DOI 10.1016/j.chroma.2017.11.009
Schönborn, Andreas, Pascal Schmid, Sarah Bräm, Georg Reifferscheid, Marina Ohlig, and Sebastian Buchinger. 2017. “Unprecedented Sensitivity of the Planar Yeast Estrogen Screen by Using a Spray-on Technology.” Journal of Chromatography A 1530 (December): 185–91. https://doi.org/10.1016/j.chroma.2017.11.009.
Schönborn, Andreas, et al. “Unprecedented Sensitivity of the Planar Yeast Estrogen Screen by Using a Spray-on Technology.” Journal of Chromatography A, vol. 1530, Dec. 2017, pp. 185–91, https://doi.org/10.1016/j.chroma.2017.11.009.
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