Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology
Authors: Schönborn, Andreas
Schmid, Pascal
Bräm, Sarah
Reifferscheid, Georg
Ohlig, Marina
Buchinger, Sebastian
DOI: 10.1016/j.chroma.2017.11.009
Published in: Journal of Chromatography A
Volume(Issue): 1530
Page(s): 185
Pages to: 191
Issue Date: 29-Dec-2017
Publisher / Ed. Institution: Elsevier
ISSN: 0021-9673
1873-3778
Language: English
Subjects: Planar-YES; Estrogenic compound; HPTLC; Hyphenation; Spray-on procedure; Sensitivity
Subject (DDC): 572: Biochemistry
Abstract: The planar yeast estrogen screen (p-YES) can serve as a highly valuable and sensitive screening tool for the detection of estrogenic compounds in various sample matrices such as water and wastewater, personal care products and foodstuff. The method combines the separation of sample constituents by thin layer chromatography with the direct detection of estrogenic compounds on the surface of the HPTLC-plate. The previous protocol using the immersion of a normal phase silica HPTLC-plate in a cell suspension for bio-autography resulted in blurred signals due to the accelerated diffusion of compounds on the wet surface of the HPTLC-plate. Here, the application of the yeast cells by spraying on the surface of the HPTLC-plate is described as an alternative approach. The presented method for the hyphenation of normal phase thin layer chromatography with a yeast estrogen screen results in much sharper signals compared to reports in previous publications. Satisfying results were achieved using cultures with cell densities of 1000 FAU. Due to the reduced signal broadening, lower limits of quantification for estrogenic compounds were achieved (Estrone (E1) = 2 pg/zone, 17β-estradiol (E2) = 0.5 pg/zone, 17α-ethinylestradiol (EE2) = 0.5 pg/zone and Estriol (E3) = 20 pg/zone). As demonstrated, it is possible to characterize profiles of estrogenic activity of wastewater samples with high quality and reproducibility. The improved sensitivity opens the stage for applications using native samples from waste- or even surface water directly applied on HPTLC-plates without the need for prior sample treatment by e.g. solid phase extraction.
URI: https://digitalcollection.zhaw.ch/handle/11475/7317
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
There are no files associated with this item.
Show full item record
Schönborn, A., Schmid, P., Bräm, S., Reifferscheid, G., Ohlig, M., & Buchinger, S. (2017). Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology. Journal of Chromatography A, 1530, 185–191. https://doi.org/10.1016/j.chroma.2017.11.009
Schönborn, A. et al. (2017) ‘Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology’, Journal of Chromatography A, 1530, pp. 185–191. Available at: https://doi.org/10.1016/j.chroma.2017.11.009.
A. Schönborn, P. Schmid, S. Bräm, G. Reifferscheid, M. Ohlig, and S. Buchinger, “Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology,” Journal of Chromatography A, vol. 1530, pp. 185–191, Dec. 2017, doi: 10.1016/j.chroma.2017.11.009.
SCHÖNBORN, Andreas, Pascal SCHMID, Sarah BRÄM, Georg REIFFERSCHEID, Marina OHLIG und Sebastian BUCHINGER, 2017. Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology. Journal of Chromatography A. 29 Dezember 2017. Bd. 1530, S. 185–191. DOI 10.1016/j.chroma.2017.11.009
Schönborn, Andreas, Pascal Schmid, Sarah Bräm, Georg Reifferscheid, Marina Ohlig, and Sebastian Buchinger. 2017. “Unprecedented Sensitivity of the Planar Yeast Estrogen Screen by Using a Spray-on Technology.” Journal of Chromatography A 1530 (December): 185–91. https://doi.org/10.1016/j.chroma.2017.11.009.
Schönborn, Andreas, et al. “Unprecedented Sensitivity of the Planar Yeast Estrogen Screen by Using a Spray-on Technology.” Journal of Chromatography A, vol. 1530, Dec. 2017, pp. 185–91, https://doi.org/10.1016/j.chroma.2017.11.009.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.