Title: Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology
Authors : Schönborn, Andreas
Schmid, Pascal
Bräm, Sarah
Reifferscheid, Georg
Ohlig, Marina
Buchinger, Sebastian
Published in : Journal of Chromatography A
Volume(Issue) : 1530
Pages : 185
Pages to: 191
Publisher / Ed. Institution : Elsevier
Issue Date: 29-Dec-2017
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Planar-YES; Estrogenic compound; HPTLC; Hyphenation; Spray-on procedure; Sensitivity
Subject (DDC) : 572: Biochemistry
Abstract: The planar yeast estrogen screen (p-YES) can serve as a highly valuable and sensitive screening tool for the detection of estrogenic compounds in various sample matrices such as water and wastewater, personal care products and foodstuff. The method combines the separation of sample constituents by thin layer chromatography with the direct detection of estrogenic compounds on the surface of the HPTLC-plate. The previous protocol using the immersion of a normal phase silica HPTLC-plate in a cell suspension for bio-autography resulted in blurred signals due to the accelerated diffusion of compounds on the wet surface of the HPTLC-plate. Here, the application of the yeast cells by spraying on the surface of the HPTLC-plate is described as an alternative approach. The presented method for the hyphenation of normal phase thin layer chromatography with a yeast estrogen screen results in much sharper signals compared to reports in previous publications. Satisfying results were achieved using cultures with cell densities of 1000 FAU. Due to the reduced signal broadening, lower limits of quantification for estrogenic compounds were achieved (Estrone (E1) = 2 pg/zone, 17β-estradiol (E2) = 0.5 pg/zone, 17α-ethinylestradiol (EE2) = 0.5 pg/zone and Estriol (E3) = 20 pg/zone). As demonstrated, it is possible to characterize profiles of estrogenic activity of wastewater samples with high quality and reproducibility. The improved sensitivity opens the stage for applications using native samples from waste- or even surface water directly applied on HPTLC-plates without the need for prior sample treatment by e.g. solid phase extraction.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Publication type: Article in scientific Journal
DOI : 10.1016/j.chroma.2017.11.009
ISSN: 0021-9673
URI: https://digitalcollection.zhaw.ch/handle/11475/7317
Appears in Collections:Publikationen Life Sciences und Facility Management

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