Title: Determination of oligomeric proanthocyanidins and their antioxidant capacity from different chocolate manufacturing stages using the NP-HPLC-online-DPPH methodology
Authors : Pedan, Vasilisa
Fischer, Norbert
Bernath, Konrad
Hühn, Tilo
Rohn, Sascha
Published in : Food Chemistry
Volume(Issue) : 214
Pages : 523
Pages to: 532
Publisher / Ed. Institution : Elsevier
Issue Date: 2017
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Antioxidant capacity; Chocolate manufacturing process; Oligomeric proanthocyanidins; Antioxidants; Cacao; Chocolate; Polyphenols; Proanthocyanidins
Subject (DDC) : 664: Food technology
Abstract: Cocoa beans are a well-known source of antioxidant polyphenols. Especially individual oligomeric proanthocyanidins demonstrated a significant contribution to the total antioxidant activity of cocoa compared to monomeric compounds. An NP-HPLC-online-DPPH assay was developed for separating the homologous series of oligomeric proanthocyanidins and the simultaneous assessment of their antioxidant capacity in relation to the degree of polymerization (DP). The present study describes the influence of the different stages of a lab-scale chocolate manufacturing process on the content of oligomeric proanthocyanidins and their antioxidant capacity. The sum of the total proanthocyanidin content (∑ DP1-DP13) decreased from 30mg epicatechin equivalents per gram non-fat dry matter in raw fresh cocoa beans to 6mg epicatechin equivalents per gram in the final chocolate. The antioxidant capacity decreased accordingly from 25mg epicatechin equivalents per gram non-fat dry matter in raw fresh cocoa beans to 4mg/g in the final chocolate product.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Food and Beverage Innovation (ILGI)
Publication type: Article in scientific Journal
DOI : 10.1016/j.foodchem.2016.07.094
ISSN: 0308-8146
URI: https://digitalcollection.zhaw.ch/handle/11475/7060
Appears in Collections:Publikationen Life Sciences und Facility Management

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