Transport through the yeast endocytic pathway occurs through morphologically distinct compartments and requires an active secretory pathway and Sec18p/N-ethylmaleimide-sensitive fusion protein

Hicke, Linda; Zanolari, Bettina; Pypaert, Marc; Rohrer, Jack; Riezman, Howard

doi:10.1091/mbc.8.1.13

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Transport through the yeast endocytic pathway occurs through morphologically distinct compartments and requires an active secretory pathway and Sec18p/N-ethylmaleimide-sensitive fusion protein
Authors:	Hicke, Linda Zanolari, Bettina Pypaert, Marc Rohrer, Jack Riezman, Howard
DOI:	10.1091/mbc.8.1.13
Published in:	Molecular Biology of the Cell
Volume(Issue):	8
Issue:	1
Page(s):	13
Pages to:	31
Issue Date:	Jan-1997
Publisher / Ed. Institution:	American Society for Cell Biology
ISSN:	1939-4586 1059-1524
Language:	English
Subject (DDC):	571: Physiology and related subjects
Abstract:	Molecules travel through the yeast endocytic pathway from the cell surface to the lysosome-like vacuole by passing through two sequential intermediates. Immunofluorescent detection of an endocytosed pheromone receptor was used to morphologically identify these intermediates, the early and late endosomes. The early endosome is a peripheral organelle that is heterogeneous in appearance, whereas the late endosome is a large perivacuolar compartment that corresponds to the prevacuolar compartment previously shown to be an endocytic intermediate. We demonstrate that inhibiting transport through the early secretory pathway in sec mutants quickly impedes transport from the early endosome. Treatment of sensitive cells with brefeldin A also blocks transport from this compartment. We provide evidence that Sec18p/N-ethylmaleimide-sensitive fusion protein, a protein required for membrane fusion, is directly required in vivo for forward transport early in the endocytic pathway. Inhibiting protein synthesis does not affect transport from the early endosome but causes endocytosed proteins to accumulate in the late endosome. As newly synthesized proteins and the late steps of secretion are not required for early to late endosome transport, but endoplasmic reticulum through Golgi traffic is, we propose that efficient forward transport in the early endocytic pathway requires delivery of lipid from secretory organelles to endosomes.
URI:	https://digitalcollection.zhaw.ch/handle/11475/6799
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Appears in collections:	Publikationen Life Sciences und Facility Management

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Hicke, L., Zanolari, B., Pypaert, M., Rohrer, J., & Riezman, H. (1997). Transport through the yeast endocytic pathway occurs through morphologically distinct compartments and requires an active secretory pathway and Sec18p/N-ethylmaleimide-sensitive fusion protein. Molecular Biology of the Cell, 8(1), 13–31. https://doi.org/10.1091/mbc.8.1.13

Hicke, L. et al. (1997) ‘Transport through the yeast endocytic pathway occurs through morphologically distinct compartments and requires an active secretory pathway and Sec18p/N-ethylmaleimide-sensitive fusion protein’, Molecular Biology of the Cell, 8(1), pp. 13–31. Available at: https://doi.org/10.1091/mbc.8.1.13.

L. Hicke, B. Zanolari, M. Pypaert, J. Rohrer, and H. Riezman, “Transport through the yeast endocytic pathway occurs through morphologically distinct compartments and requires an active secretory pathway and Sec18p/N-ethylmaleimide-sensitive fusion protein,” Molecular Biology of the Cell, vol. 8, no. 1, pp. 13–31, Jan. 1997, doi: 10.1091/mbc.8.1.13.

HICKE, Linda, Bettina ZANOLARI, Marc PYPAERT, Jack ROHRER und Howard RIEZMAN, 1997. Transport through the yeast endocytic pathway occurs through morphologically distinct compartments and requires an active secretory pathway and Sec18p/N-ethylmaleimide-sensitive fusion protein. Molecular Biology of the Cell. Januar 1997. Bd. 8, Nr. 1, S. 13–31. DOI 10.1091/mbc.8.1.13

Hicke, Linda, Bettina Zanolari, Marc Pypaert, Jack Rohrer, and Howard Riezman. 1997. “Transport through the Yeast Endocytic Pathway Occurs through Morphologically Distinct Compartments and Requires an Active Secretory Pathway and Sec18p/N-Ethylmaleimide-Sensitive Fusion Protein.” Molecular Biology of the Cell 8 (1): 13–31. https://doi.org/10.1091/mbc.8.1.13.

Hicke, Linda, et al. “Transport through the Yeast Endocytic Pathway Occurs through Morphologically Distinct Compartments and Requires an Active Secretory Pathway and Sec18p/N-Ethylmaleimide-Sensitive Fusion Protein.” Molecular Biology of the Cell, vol. 8, no. 1, Jan. 1997, pp. 13–31, https://doi.org/10.1091/mbc.8.1.13.