Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4220
Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Antioxidant generation during coffee roasting : a comparison and interpretation from three complementary assays
Authors: Opitz, Sebastian
Smrke, Samo
Goodman, Bernard
Keller, Marco
Schenker, Stefan
Yeretzian, Chahan
DOI: 10.21256/zhaw-4220
10.3390/foods3040586
Published in: Foods
Volume(Issue): 3
Issue: 4
Page(s): 586
Pages to: 604
Issue Date: 2014
Publisher / Ed. Institution: MDPI
ISSN: 2304-8158
Language: English
Subjects: Roast parameter; Coffee; Antioxidant assays
Subject (DDC): 663: Beverage technology
Abstract: Coffee is a major source of dietary antioxidants; some are present in the green bean, whereas others are generated during roasting. However, there is no single accepted analytical method for their routine determination. This paper describes the adaption of three complementary assays (Folin-Ciocalteu (FC), ABTS and ORAC) for the routine assessment of antioxidant capacity of beverages, their validation, and use for determining the antioxidant capacities of extracts from coffee beans at different stages in the roasting process. All assays showed a progressive increase in antioxidant capacity during roasting to a light roast state, consistent with the production of melanoidins having a higher antioxidant effect than the degradation of CGAs. However, the three assays gave different numbers for the total antioxidant capacity of green beans relative to gallic acid (GA), although the range of values was much smaller when chlorogenic acid (CGA) was used as reference. Therefore, although all three assays indicated that there was an increase in antioxidant activity during coffee roasting, and the large differences in responses to GA and CGA illustrate their different sensitivities to different types of antioxidant molecule.
URI: https://digitalcollection.zhaw.ch/handle/11475/5550
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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Opitz, S., Smrke, S., Goodman, B., Keller, M., Schenker, S., & Yeretzian, C. (2014). Antioxidant generation during coffee roasting : a comparison and interpretation from three complementary assays. Foods, 3(4), 586–604. https://doi.org/10.21256/zhaw-4220
Opitz, S. et al. (2014) ‘Antioxidant generation during coffee roasting : a comparison and interpretation from three complementary assays’, Foods, 3(4), pp. 586–604. Available at: https://doi.org/10.21256/zhaw-4220.
S. Opitz, S. Smrke, B. Goodman, M. Keller, S. Schenker, and C. Yeretzian, “Antioxidant generation during coffee roasting : a comparison and interpretation from three complementary assays,” Foods, vol. 3, no. 4, pp. 586–604, 2014, doi: 10.21256/zhaw-4220.
OPITZ, Sebastian, Samo SMRKE, Bernard GOODMAN, Marco KELLER, Stefan SCHENKER und Chahan YERETZIAN, 2014. Antioxidant generation during coffee roasting : a comparison and interpretation from three complementary assays. Foods. 2014. Bd. 3, Nr. 4, S. 586–604. DOI 10.21256/zhaw-4220
Opitz, Sebastian, Samo Smrke, Bernard Goodman, Marco Keller, Stefan Schenker, and Chahan Yeretzian. 2014. “Antioxidant Generation during Coffee Roasting : A Comparison and Interpretation from Three Complementary Assays.” Foods 3 (4): 586–604. https://doi.org/10.21256/zhaw-4220.
Opitz, Sebastian, et al. “Antioxidant Generation during Coffee Roasting : A Comparison and Interpretation from Three Complementary Assays.” Foods, vol. 3, no. 4, 2014, pp. 586–604, https://doi.org/10.21256/zhaw-4220.


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