Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-2679
Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Quantitative PCR assay for detection of bois noir phytoplasmas in grape and insect tissue
Authors: Fahrentrapp, Johannes
Michl, Gertraud
Breuer, Michael
DOI: 10.21256/zhaw-2679
Published in: Vitis - Journal of Grapevine Research
Volume(Issue): 52
Issue: 2
Pages: 85
Pages to: 89
Issue Date: 2013
Publisher / Ed. Institution: Julius Kühn Institute for Grapevine Breeding
ISSN: 0042-7500
2367-4156
Language: English
Subjects: Phytoplasma disease; Quantitative real-time PCR (qPCR); "Vergilbungskrankheit"; Grape disease
Subject (DDC): 572: Biochemistry
634: Orchards, fruits and forestry
Abstract: In Europe's vineyards "Bois noir" (BN) is an expanding yellows disease on Vitis vinifera. It is associated with phytoplasmas of the stolbur group (16SrXII-A). Two subtypes are important, one is associated with Urtica dioica and one with Convolvulus arvensis. Both phytoplasma types are transmitted by the insect Hyalesthes obsoletus. A nucleic acid extraction method for V. vinifera and H. obsoletus was developed together with a real time PCR (qPCR) assay based on a polymorphic sequence with homology to a putative dimethyladenosine transferase. The comparison of the conventional detection method with the qPCR assay of 40 insect and 40 V. vinifera samples showed a 10% higher sensitivity of qPCR in plant samples. The titer of phytoplasmas in H. obsoletus was 2643-fold increased in the strongest infected samples compared to the lowest ones. The results suggest this real-time PCR as a valid and fast alternative procedure for the detection and quantification of BN phytoplasmas. The assay allows to discriminate the two phytoplasma types and to quantify phytoplasmas in H. obsoletus.
URI: https://digitalcollection.zhaw.ch/handle/11475/4096
Fulltext version: Published version
License (according to publishing contract): CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project: Detection of Bois noir phytoplasma in the vectoring ciciid Hyalethes obsoletus.
Appears in Collections:Publikationen Life Sciences und Facility Management

Files in This Item:
File Description SizeFormat 
2013_Fahrentrapp_Quantitative PCR assay_VITIS.pdf525.04 kBAdobe PDFThumbnail
View/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.