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Title: Quantitative PCR assay for detection of bois noir phytoplasmas in grape and insect tissue
Authors : Fahrentrapp, Johannes
Michl, Gertraud
Breuer, Michael
Published in : VITIS: Journal of Grapevine Research
Volume(Issue) : 52
Issue : 2
Pages : 85
Pages to: 89
Publisher / Ed. Institution : Julius Kühn-Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Rebenzüchtung, Geilweilerhof
Issue Date: 2013
License (according to publishing contract) : CC BY 4.0: Attribution 4.0 International
Type of review: Peer review (Publication)
Language : English
Subjects : Phytoplasma disease; Quantitative real-time PCR (qPCR); "Vergilbungskrankheit"; Grape disease
Subject (DDC) : 572: Biochemistry
634: Orchards, fruits and forestry
Abstract: In Europe's vineyards "Bois noir" (BN) is an expanding yellows disease on Vitis vinifera. It is associated with phytoplasmas of the stolbur group (16SrXII-A). Two subtypes are important, one is associated with Urtica dioica and one with Convolvulus arvensis. Both phytoplasma types are transmitted by the insect Hyalesthes obsoletus. A nucleic acid extraction method for V. vinifera and H. obsoletus was developed together with a real time PCR (qPCR) assay based on a polymorphic sequence with homology to a putative dimethyladenosine transferase. The comparison of the conventional detection method with the qPCR assay of 40 insect and 40 V. vinifera samples showed a 10 % higher sensitivity of qPCR in plant samples. The titer of phytoplasmas in H. obsoletus was 2643-fold increased in the strongest infected samples compared to the lowest ones. The results suggest this real-time PCR as a valid and fast alternative procedure for the detection and quantification of BN phytoplasmas. The assay allows to discriminate the two phytoplasma types and to quantify phytoplasmas in H. obsoletus.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Publication type: Article in scientific Journal
DOI : 10.21256/zhaw-2679
ISSN: 0042-7500
Published as part of the ZHAW project : Detection of Bois noir phytoplasma in the vectoring ciciid Hyalethes obsoletus.
Appears in Collections:Publikationen Life Sciences und Facility Management

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