| Publication type: | Book part |
| Type of review: | Editorial review |
| Title: | Chemically defined, xeno-free expansion of human mesenchymal stem cells (hMSCs) on benchtop-scale using a stirred single-use bioreactor |
| Authors: | Teale, Misha Jossen, Valentin Eibl, Dieter Eibl-Schindler, Regine |
| et. al: | No |
| DOI: | 10.1007/7651_2021_426 |
| Published in: | Bioreactors in Stem Cell Biology : Methods and Protocols |
| Editors of the parent work: | Turksen, Kursad |
| Page(s): | 83 |
| Pages to: | 111 |
| Issue Date: | 6-Oct-2021 |
| Series: | Methods in Molecular Biology |
| Series volume: | 2436 |
| Publisher / Ed. Institution: | Humana |
| Publisher / Ed. Institution: | New York, NY |
| ISBN: | 978-1-0716-2017-5 978-1-0716-2018-2 |
| Language: | English |
| Subjects: | BioBLU® 0.3c bioreactor; Cell expansion; Microcarrier; UrSuppe; hTERT immortalized ASC5Telo cell; Single-use |
| Subject (DDC): | 660: Chemical engineering |
| Abstract: | In recent years, the use of hMSCs, which may be isolated from adipose tissue among others, for the treatment of diseases has increased significantly. The cell quantities required for such therapeutic approaches, between 1012 and 1013, have thus far been predominantly produced using commercially available multi-tray systems, such as the Cell Factory (Thermo Fisher Scientific) or HYPERStack (Corning), which can be purchased with up to 40 layers. However, the handling of these planar multilayer systems is difficult, and process monitoring opportunities remain limited. Here, automated stirred single-use bioreactors provide a viable alternative to the time-consuming multiplication of cells using such planar systems, while still managing to achieve the desired clinically relevant quantities. In these stirred single-use systems, adherent cells are predominantly cultivated in suspension up to pilot scale using carrier materials, also referred to as microcarriers (MCs).This chapter describes the steps which need to be realized to guarantee successful hMSC expansion within a stirred single-use bioreactor (Eppendorf's BioBLU® 0.3c) operated using MCs under serum- and xeno-free conditions at benchtop scale. The cultivations were performed using an immortalized human adipose-derived mesenchymal stem cell (hASC) line, hence referred to as hASC52telo, and a new chemically defined, xeno-free medium, hence referred to as the UrSuppe formulation. Spinner flask cultivations were performed under comparable process conditions. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/30295 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
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Teale, M., Jossen, V., Eibl, D., & Eibl-Schindler, R. (2021). Chemically defined, xeno-free expansion of human mesenchymal stem cells (hMSCs) on benchtop-scale using a stirred single-use bioreactor. In K. Turksen (Ed.), Bioreactors in Stem Cell Biology : Methods and Protocols (pp. 83–111). Humana. https://doi.org/10.1007/7651_2021_426
Teale, M. et al. (2021) ‘Chemically defined, xeno-free expansion of human mesenchymal stem cells (hMSCs) on benchtop-scale using a stirred single-use bioreactor’, in K. Turksen (ed.) Bioreactors in Stem Cell Biology : Methods and Protocols. New York, NY: Humana, pp. 83–111. Available at: https://doi.org/10.1007/7651_2021_426.
M. Teale, V. Jossen, D. Eibl, and R. Eibl-Schindler, “Chemically defined, xeno-free expansion of human mesenchymal stem cells (hMSCs) on benchtop-scale using a stirred single-use bioreactor,” in Bioreactors in Stem Cell Biology : Methods and Protocols, K. Turksen, Ed. New York, NY: Humana, 2021, pp. 83–111. doi: 10.1007/7651_2021_426.
TEALE, Misha, Valentin JOSSEN, Dieter EIBL und Regine EIBL-SCHINDLER, 2021. Chemically defined, xeno-free expansion of human mesenchymal stem cells (hMSCs) on benchtop-scale using a stirred single-use bioreactor. In: Kursad TURKSEN (Hrsg.), Bioreactors in Stem Cell Biology : Methods and Protocols. New York, NY: Humana. S. 83–111. ISBN 978-1-0716-2017-5
Teale, Misha, Valentin Jossen, Dieter Eibl, and Regine Eibl-Schindler. 2021. “Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor.” In Bioreactors in Stem Cell Biology : Methods and Protocols, edited by Kursad Turksen, 83–111. New York, NY: Humana. https://doi.org/10.1007/7651_2021_426.
Teale, Misha, et al. “Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor.” Bioreactors in Stem Cell Biology : Methods and Protocols, edited by Kursad Turksen, Humana, 2021, pp. 83–111, https://doi.org/10.1007/7651_2021_426.
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