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https://doi.org/10.21256/zhaw-30137| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Process intensification in human pluripotent stem cell expansion with microcarriers |
| Authors: | Teale, Misha Alexander Schneider, Samuel Lukas Eibl, Dieter Eibl-Schindler, Regine |
| et. al: | No |
| DOI: | 10.3390/pr12030426 10.21256/zhaw-30137 |
| Published in: | Processes |
| Volume(Issue): | 12 |
| Issue: | 3 |
| Page(s): | 426 |
| Issue Date: | 20-Feb-2024 |
| Publisher / Ed. Institution: | MDPI |
| ISSN: | 2227-9717 |
| Language: | English |
| Subjects: | Microcarriers; Perfusion; Single-use; Stirred bioreactor; Upstream processing |
| Subject (DDC): | 660.6: Biotechnology |
| Abstract: | Given the demands human induced pluripotent stem cell (hiPSC)-based therapeutics place on manufacturing, process intensification strategies which rapidly ensure the desired cell quality and quantity should be considered. Within the context of antibody and vaccine manufacturing, one-step inoculation has emerged as an effective strategy for intensifying the upstream process. This study therefore evaluated whether this approach could be applied to the expansion of hiPSCs in flasks under static and in microcarrier-operated stirred bioreactors under dynamic conditions. Our findings demonstrated that high density working cell banks containing hiPSCs at concentrations of up to 100 × 106 cells mL−1 in CryoStor® CS10 did not impair cell growth and quality upon thawing. Furthermore, while cell distribution, growth, and viability were comparable to routinely passaged hiPSCs, those subjected to one-step inoculation and expansion on microcarriers under stirred conditions were characterized by improved attachment efficiency (≈50%) following the first day of cultivation. Accordingly, the process development outlined in this study establishes the foundation for the implementation of this intensified approach at L-scale. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/30137 |
| Fulltext version: | Published version |
| License (according to publishing contract): | CC BY 4.0: Attribution 4.0 International |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2024_Teale-etal_Process-human-stemm-cell-expansion_MDPI.pdf | 6.27 MB | Adobe PDF |  View/Open |
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Teale, M. A., Schneider, S. L., Eibl, D., & Eibl-Schindler, R. (2024). Process intensification in human pluripotent stem cell expansion with microcarriers. Processes, 12(3), 426. https://doi.org/10.3390/pr12030426
Teale, M.A. et al. (2024) ‘Process intensification in human pluripotent stem cell expansion with microcarriers’, Processes, 12(3), p. 426. Available at: https://doi.org/10.3390/pr12030426.
M. A. Teale, S. L. Schneider, D. Eibl, and R. Eibl-Schindler, “Process intensification in human pluripotent stem cell expansion with microcarriers,” Processes, vol. 12, no. 3, p. 426, Feb. 2024, doi: 10.3390/pr12030426.
TEALE, Misha Alexander, Samuel Lukas SCHNEIDER, Dieter EIBL und Regine EIBL-SCHINDLER, 2024. Process intensification in human pluripotent stem cell expansion with microcarriers. Processes. 20 Februar 2024. Bd. 12, Nr. 3, S. 426. DOI 10.3390/pr12030426
Teale, Misha Alexander, Samuel Lukas Schneider, Dieter Eibl, and Regine Eibl-Schindler. 2024. “Process Intensification in Human Pluripotent Stem Cell Expansion with Microcarriers.” Processes 12 (3): 426. https://doi.org/10.3390/pr12030426.
Teale, Misha Alexander, et al. “Process Intensification in Human Pluripotent Stem Cell Expansion with Microcarriers.” Processes, vol. 12, no. 3, Feb. 2024, p. 426, https://doi.org/10.3390/pr12030426.
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