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https://doi.org/10.21256/zhaw-30135| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Process intensification by inoculating antibody production bioreactors directly from cryovials |
| Authors: | Ott, Jan Ott, Vivian Neubauer, Peter Eibl, Dieter Eibl-Schindler, Regine |
| et. al: | No |
| DOI: | 10.1002/cite.202300189 10.21256/zhaw-30135 |
| Published in: | Chemie Ingenieur Technik |
| Volume(Issue): | 96 |
| Issue: | 4 |
| Page(s): | 453 |
| Pages to: | 461 |
| Issue Date: | Mar-2024 |
| Publisher / Ed. Institution: | Wiley |
| ISSN: | 0009-286X 1522-2640 |
| Language: | English |
| Subjects: | Chinese hamster ovary cells; Fed-batch; Perfusion; 1:10 Turndown ratio; Ultra-high cell density cryovial; Antibody |
| Subject (DDC): | 660: Chemical engineering |
| Abstract: | There are several ways to make antibody production processes more efficient and less expensive through process intensification. In this study, an ultra-high cell density working cell bank was used to inoculate production bioreactors directly with cells from cryovials. After lab-scale experiments, a fed-batch pilot-scale experiment was successfully conducted as a proof-of-concept. In addition, cells were cultivated in perfusion mode at 2 L scale. The intensification approach presented here allows the complete elimination of the inoculum production outside the production bioreactor and the creation of additional production capacity by saving time and space. In perfusion mode, an increase in bioreactor productivity of over 180% can be achieved compared to fed-batch mode. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/30135 |
| Fulltext version: | Published version |
| License (according to publishing contract): | CC BY 4.0: Attribution 4.0 International |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| 2024_Ott-etal_Process-intensification-by-inoculating-antibody-production-bioreactors.pdf | 704.87 kB | Adobe PDF |  View/Open |
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Ott, J., Ott, V., Neubauer, P., Eibl, D., & Eibl-Schindler, R. (2024). Process intensification by inoculating antibody production bioreactors directly from cryovials . Chemie Ingenieur Technik, 96(4), 453–461. https://doi.org/10.1002/cite.202300189
Ott, J. et al. (2024) ‘Process intensification by inoculating antibody production bioreactors directly from cryovials ’, Chemie Ingenieur Technik, 96(4), pp. 453–461. Available at: https://doi.org/10.1002/cite.202300189.
J. Ott, V. Ott, P. Neubauer, D. Eibl, and R. Eibl-Schindler, “Process intensification by inoculating antibody production bioreactors directly from cryovials ,” Chemie Ingenieur Technik, vol. 96, no. 4, pp. 453–461, Mar. 2024, doi: 10.1002/cite.202300189.
OTT, Jan, Vivian OTT, Peter NEUBAUER, Dieter EIBL und Regine EIBL-SCHINDLER, 2024. Process intensification by inoculating antibody production bioreactors directly from cryovials . Chemie Ingenieur Technik. März 2024. Bd. 96, Nr. 4, S. 453–461. DOI 10.1002/cite.202300189
Ott, Jan, Vivian Ott, Peter Neubauer, Dieter Eibl, and Regine Eibl-Schindler. 2024. “Process Intensification by Inoculating Antibody Production Bioreactors Directly from Cryovials .” Chemie Ingenieur Technik 96 (4): 453–61. https://doi.org/10.1002/cite.202300189.
Ott, Jan, et al. “Process Intensification by Inoculating Antibody Production Bioreactors Directly from Cryovials .” Chemie Ingenieur Technik, vol. 96, no. 4, Mar. 2024, pp. 453–61, https://doi.org/10.1002/cite.202300189.
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