Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics

Kałużna, Monika; Prokić, Andjelka; Obradović, Aleksa; Weldon, William A.; Stockwell, Virginia O.; Pothier, Joël F.

doi:10.3389/fpls.2023.1254107

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-28702

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics
Authors:	Kałużna, Monika Prokić, Andjelka Obradović, Aleksa Weldon, William A. Stockwell, Virginia O. Pothier, Joël F.
et. al:	No
DOI:	10.3389/fpls.2023.1254107 10.21256/zhaw-28702
Published in:	Frontiers in Plant Science
Volume(Issue):	14
Issue:	1254107
Issue Date:	13-Sep-2023
Publisher / Ed. Institution:	Frontiers Research Foundation
ISSN:	1664-462X
Language:	English
Subjects:	Corylus spp.; Corylus avellana; Diagnosis; PCR; LAMP; qPCR
Subject (DDC):	572: Biochemistry 579: Microbiology
Abstract:	Xanthomonas arboricola pv. corylina (Xac; formerly Xanthomonas campestris pv. corylina) is the causal agent of the bacterial blight of hazelnuts, a devastating disease of trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars of X. arboricola. A comparative genomics approach with publicly available genomes of Xac was used to identify unique sequences, conserved across the genomes of the pathogen. We identified a 2,440 bp genomic region that was unique to Xac and designed identification and detection systems for conventional PCR, qPCR (SYBR® Green and TaqMan™), and loop-mediated isothermal amplification (LAMP). All PCR assays performed on genomic DNA isolated from eight X. arboricola pathovars and closely related bacterial species confirmed the specificity of designed primers. These new multi-platform molecular diagnostic tools may be used by plant clinics and researchers to detect and identify Xac in pure cultures and hazelnut tissues rapidly and accurately.
URI:	https://digitalcollection.zhaw.ch/handle/11475/28702
Related research data:	https://www.ebi.ac.uk/ena/browser/view/PRJEB42844
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project:	Comparative genomics of the plant pathogen Xanthomonas arboricola pv. pruni and related xanthomonads
Appears in collections:	Publikationen Life Sciences und Facility Management

Files in This Item:

File	Description	Size	Format
2023_Kaluzna-etal_Xanthomonas-arboricola-pv-corylina-detection-tools.pdf	Article	1.11 MB	Adobe PDF	View/Open
2023_Kaluzna-etal_Xanthomonas-arboricola-pv-corylina-detection-tools_Suppl.pdf	Supplementary Material	793.68 kB	Adobe PDF	View/Open

Show full item record

Kałużna, M., Prokić, A., Obradović, A., Weldon, W. A., Stockwell, V. O., & Pothier, J. F. (2023). Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics. Frontiers in Plant Science, 14(1254107). https://doi.org/10.3389/fpls.2023.1254107

Kałużna, M. et al. (2023) ‘Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics’, Frontiers in Plant Science, 14(1254107). Available at: https://doi.org/10.3389/fpls.2023.1254107.

M. Kałużna, A. Prokić, A. Obradović, W. A. Weldon, V. O. Stockwell, and J. F. Pothier, “Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics,” Frontiers in Plant Science, vol. 14, no. 1254107, Sep. 2023, doi: 10.3389/fpls.2023.1254107.

KAŁUŻNA, Monika, Andjelka PROKIĆ, Aleksa OBRADOVIĆ, William A. WELDON, Virginia O. STOCKWELL und Joël F. POTHIER, 2023. Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics. Frontiers in Plant Science. 13 September 2023. Bd. 14, Nr. 1254107. DOI 10.3389/fpls.2023.1254107

Kałużna, Monika, Andjelka Prokić, Aleksa Obradović, William A. Weldon, Virginia O. Stockwell, and Joël F. Pothier. 2023. “Specific and Sensitive Detection Tools for Xanthomonas Arboricola Pv. Corylina, the Causal Agent of Bacterial Blight of Hazelnut, Developed with Comparative Genomics.” Frontiers in Plant Science 14 (1254107). https://doi.org/10.3389/fpls.2023.1254107.

Kałużna, Monika, et al. “Specific and Sensitive Detection Tools for Xanthomonas Arboricola Pv. Corylina, the Causal Agent of Bacterial Blight of Hazelnut, Developed with Comparative Genomics.” Frontiers in Plant Science, vol. 14, no. 1254107, Sept. 2023, https://doi.org/10.3389/fpls.2023.1254107.