Excess polyspermy reduces the ability of porcine oocytes to promote male pronuclear formation after in vitro fertilization

Nguyen, Hiep Thi; Dang-Nguyen, Thanh Quang; Somfai, Tamas; Men, Nguyen Thi; Beck-Woerner, Barbara; Viet Linh, Nguyen; Xuan Nguyen, Bui; Noguchi, Junko; Kaneko, Hiroyuki; Kikuchi, Kazuhiro

doi:10.1111/asj.13650

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-28381

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Excess polyspermy reduces the ability of porcine oocytes to promote male pronuclear formation after in vitro fertilization
Authors:	Nguyen, Hiep Thi Dang-Nguyen, Thanh Quang Somfai, Tamas Men, Nguyen Thi Beck-Woerner, Barbara Viet Linh, Nguyen Xuan Nguyen, Bui Noguchi, Junko Kaneko, Hiroyuki Kikuchi, Kazuhiro
et. al:	No
DOI:	10.1111/asj.13650 10.21256/zhaw-28381
Published in:	Animal Science Journal
Volume(Issue):	92
Issue:	1
Page(s):	e13650
Issue Date:	26-Oct-2021
Publisher / Ed. Institution:	Wiley
ISSN:	1740-0929
Language:	English
Subjects:	IVF; Male pronucleus formation; Pig; polyspermy; Fertilization; Oocytes; Spermatozoa; Swine; Fertilization in vitro; Sperm-ovum interactions
Subject (DDC):	571: Physiology and related subjects
Abstract:	Male pronucleus (MPN) formation is a very important physiological event during fertilization, which affects in vitro production of transferrable embryos. The aim of this study was to find out the correlation between the number of penetrated sperm and the occurrence of failure of MPN formation in porcine oocytes. In vitro matured porcine oocytes were fertilized in vitro with frozen epididymal sperm. Two different frozen sperm lots were tested in this study, which were different in terms of polyspermy rates. The numbers and the status of penetrated sperm in oocytes were evaluated 10 h after insemination. Under high polyspermy condition, the polyspermy rate was 83.5% with an average mean of 3.5 sperms per penetrated oocyte, whereas the percentage of polyspermy was 65.5% with an average mean of 2.4 sperms per penetrated oocyte under moderate polyspermic condition. Correlation analysis revealed a negative correlation between the number of penetrated sperm and their MPN formation percentage both in the sperm lot of high polyspermy (R = -0.560, p < 0.05) and in the sperm lot of moderate polyspermy (R = -0.405, p < 0.05) which suggests that penetration of excessive spermatozoa disables the oocyte cytoplasm to promote MPN formation.
URI:	https://digitalcollection.zhaw.ch/handle/11475/28381
Fulltext version:	Published version
License (according to publishing contract):	CC BY-NC 4.0: Attribution - Non commercial 4.0 International
Departement:	Life Sciences and Facility Management
Appears in collections:	Publikationen Life Sciences und Facility Management

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Nguyen, H. T., Dang-Nguyen, T. Q., Somfai, T., Men, N. T., Beck-Woerner, B., Viet Linh, N., Xuan Nguyen, B., Noguchi, J., Kaneko, H., & Kikuchi, K. (2021). Excess polyspermy reduces the ability of porcine oocytes to promote male pronuclear formation after in vitro fertilization. Animal Science Journal, 92(1), e13650. https://doi.org/10.1111/asj.13650

Nguyen, H.T. et al. (2021) ‘Excess polyspermy reduces the ability of porcine oocytes to promote male pronuclear formation after in vitro fertilization’, Animal Science Journal, 92(1), p. e13650. Available at: https://doi.org/10.1111/asj.13650.

H. T. Nguyen et al., “Excess polyspermy reduces the ability of porcine oocytes to promote male pronuclear formation after in vitro fertilization,” Animal Science Journal, vol. 92, no. 1, p. e13650, Oct. 2021, doi: 10.1111/asj.13650.

NGUYEN, Hiep Thi, Thanh Quang DANG-NGUYEN, Tamas SOMFAI, Nguyen Thi MEN, Barbara BECK-WOERNER, Nguyen VIET LINH, Bui XUAN NGUYEN, Junko NOGUCHI, Hiroyuki KANEKO und Kazuhiro KIKUCHI, 2021. Excess polyspermy reduces the ability of porcine oocytes to promote male pronuclear formation after in vitro fertilization. Animal Science Journal. 26 Oktober 2021. Bd. 92, Nr. 1, S. e13650. DOI 10.1111/asj.13650

Nguyen, Hiep Thi, Thanh Quang Dang-Nguyen, Tamas Somfai, Nguyen Thi Men, Barbara Beck-Woerner, Nguyen Viet Linh, Bui Xuan Nguyen, Junko Noguchi, Hiroyuki Kaneko, and Kazuhiro Kikuchi. 2021. “Excess Polyspermy Reduces the Ability of Porcine Oocytes to Promote Male Pronuclear Formation after in Vitro Fertilization.” Animal Science Journal 92 (1): e13650. https://doi.org/10.1111/asj.13650.

Nguyen, Hiep Thi, et al. “Excess Polyspermy Reduces the Ability of Porcine Oocytes to Promote Male Pronuclear Formation after in Vitro Fertilization.” Animal Science Journal, vol. 92, no. 1, Oct. 2021, p. e13650, https://doi.org/10.1111/asj.13650.