Race-specific genotypes of Pseudomonas syringae pv. tomato are defined by the presence of mobile DNA elements within the genome

Orfei, Benedetta; Pothier, Joël F.; Fenske, Linda; Blom, Jochen; Moretti, Chiaraluce; Buonaurio, Roberto; Smits, Theo H. M.

doi:10.3389/fpls.2023.1197706

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-28289

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Race-specific genotypes of Pseudomonas syringae pv. tomato are defined by the presence of mobile DNA elements within the genome
Authors:	Orfei, Benedetta Pothier, Joël F. Fenske, Linda Blom, Jochen Moretti, Chiaraluce Buonaurio, Roberto Smits, Theo H. M.
et. al:	No
DOI:	10.3389/fpls.2023.1197706 10.21256/zhaw-28289
Published in:	Frontiers in Plant Science
Volume(Issue):	14
Issue:	1197706
Issue Date:	5-Jul-2023
Publisher / Ed. Institution:	Frontiers Research Foundation
ISSN:	1664-462X
Language:	English
Subjects:	Pseudomonas avellanae; Pseudomonas syringae pv tomato; Comparative genomics; AvrPto1; AvrPtoB; Race shift
Subject (DDC):	572: Biochemistry 579: Microbiology
Abstract:	Pseudomonas syringae pv. tomato is the causal agent of bacterial speck of tomato, an important disease that results in severe crop production losses worldwide. Currently, two races within phylogroup 01a (PG01a) are described for this pathogen. Race 0 strains have avirulence genes for the expression of type III system-associated effectors AvrPto1 and AvrPtoB, that are recognized and targeted by the effector-triggered immunity in tomato cultivars having the pto race-specific resistance gene. Race 1 strains instead lack the avrPto1 and avrPtoB genes and are therefore capable to aggressively attack all tomato cultivars. Here, we have performed the complete genome sequencing and the analysis of P. syringae pv. tomato strain DAPP-PG 215, which was described as a race 0 strain in 1996. Our analysis revealed that its genome comprises a 6.2 Mb circular chromosome and two plasmids (107 kb and 81 kb). The results indicate that the strain is phylogenetically closely related to strains Max13, K40, T1 and NYS-T1, all known race 1 strains. The chromosome of DAPP-PG 215 encodes race 1-associated genes like avrA and hopW1 and lacks race 0-associated genes like hopN1, giving it a race 1 genetic background. However, the genome harbors a complete ortholog of avrPto1, which allows the strain to display a race 0 phenotype. Comparative genomics with several PG01a genomes revealed that mobile DNA elements are rather involved in the evolution of the two different races.
URI:	https://digitalcollection.zhaw.ch/handle/11475/28289
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Orfei, B., Pothier, J. F., Fenske, L., Blom, J., Moretti, C., Buonaurio, R., & Smits, T. H. M. (2023). Race-specific genotypes of Pseudomonas syringae pv. tomato are defined by the presence of mobile DNA elements within the genome. Frontiers in Plant Science, 14(1197706). https://doi.org/10.3389/fpls.2023.1197706

Orfei, B. et al. (2023) ‘Race-specific genotypes of Pseudomonas syringae pv. tomato are defined by the presence of mobile DNA elements within the genome’, Frontiers in Plant Science, 14(1197706). Available at: https://doi.org/10.3389/fpls.2023.1197706.

B. Orfei et al., “Race-specific genotypes of Pseudomonas syringae pv. tomato are defined by the presence of mobile DNA elements within the genome,” Frontiers in Plant Science, vol. 14, no. 1197706, Jul. 2023, doi: 10.3389/fpls.2023.1197706.

ORFEI, Benedetta, Joël F. POTHIER, Linda FENSKE, Jochen BLOM, Chiaraluce MORETTI, Roberto BUONAURIO und Theo H. M. SMITS, 2023. Race-specific genotypes of Pseudomonas syringae pv. tomato are defined by the presence of mobile DNA elements within the genome. Frontiers in Plant Science. 5 Juli 2023. Bd. 14, Nr. 1197706. DOI 10.3389/fpls.2023.1197706

Orfei, Benedetta, Joël F. Pothier, Linda Fenske, Jochen Blom, Chiaraluce Moretti, Roberto Buonaurio, and Theo H. M. Smits. 2023. “Race-Specific Genotypes of Pseudomonas Syringae Pv. Tomato Are Defined by the Presence of Mobile DNA Elements within the Genome.” Frontiers in Plant Science 14 (1197706). https://doi.org/10.3389/fpls.2023.1197706.

Orfei, Benedetta, et al. “Race-Specific Genotypes of Pseudomonas Syringae Pv. Tomato Are Defined by the Presence of Mobile DNA Elements within the Genome.” Frontiers in Plant Science, vol. 14, no. 1197706, July 2023, https://doi.org/10.3389/fpls.2023.1197706.