Improvement of HEK293 cell growth by adapting hydrodynamic stress and predicting cell aggregate size distribution

Seidel, Stefan; Maschke, Rüdiger; Mozaffari, Fruhar; Eibl-Schindler, Regine; Eibl, Dieter

doi:10.3390/bioengineering10040478

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-27766

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Improvement of HEK293 cell growth by adapting hydrodynamic stress and predicting cell aggregate size distribution
Authors:	Seidel, Stefan Maschke, Rüdiger Mozaffari, Fruhar Eibl-Schindler, Regine Eibl, Dieter
et. al:	No
DOI:	10.3390/bioengineering10040478 10.21256/zhaw-27766
Published in:	Bioengineering
Volume(Issue):	10
Issue:	4
Page(s):	478
Issue Date:	16-Apr-2023
Publisher / Ed. Institution:	MDPI
ISSN:	2306-5354
Language:	English
Subjects:	Aggregate size distribution; Biochemical engineering; CFD computational fluid dynamics; Energy dissipation rate; Fluid dynamic stress; HEK293 suspension culture; Kolmogorov length
Subject (DDC):	660.6: Biotechnology
Abstract:	HEK293 is a widely used cell line in the fields of research and industry. It is assumed that these cells are sensitive to hydrodynamic stress. The aim of this research was to use particle image velocimetry validated computational fluid dynamics (CFD) to determine the hydrodynamic stress in both shake flasks, with and without baffles, and in stirred Minifors 2 bioreactors to evaluate its effect on the growth and aggregate size distribution of HEK293 suspension cells. The HEK FreeStyle™ 293-F cell line was cultivated in batch mode at different specific power inputs (from 63 W/m³ to 451 W/m³), whereby approx. 60 W/m³ corresponds to the upper limit, which is what has been typically described in published experiments. In addition to the specific growth rate and maximum viable cell density VCDmax, the cell size distribution over time and cluster size distribution were investigated. The VCDmax of (5.77 ± 0.02) · 10⁶ cells/mL was reached at a specific power input of 233 W/m³ and was 23.8% higher than the value obtained at 63 W/m³ and 7.2% higher than the value obtained at 451 W/m³. No significant change in the cell size distribution could be measured in the investigated range. It was shown that the cell cluster size distribution follows a strict geometric distribution whose free parameter p is linearly dependent on the mean Kolmogorov length scale. Based on the performed experiments, it has been shown that by using CFD-characterised bioreactors, the VCDmax can be increased and the cell aggregate rate can be precisely controlled.
URI:	https://digitalcollection.zhaw.ch/handle/11475/27766
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Seidel, S., Maschke, R., Mozaffari, F., Eibl-Schindler, R., & Eibl, D. (2023). Improvement of HEK293 cell growth by adapting hydrodynamic stress and predicting cell aggregate size distribution. Bioengineering, 10(4), 478. https://doi.org/10.3390/bioengineering10040478

Seidel, S. et al. (2023) ‘Improvement of HEK293 cell growth by adapting hydrodynamic stress and predicting cell aggregate size distribution’, Bioengineering, 10(4), p. 478. Available at: https://doi.org/10.3390/bioengineering10040478.

S. Seidel, R. Maschke, F. Mozaffari, R. Eibl-Schindler, and D. Eibl, “Improvement of HEK293 cell growth by adapting hydrodynamic stress and predicting cell aggregate size distribution,” Bioengineering, vol. 10, no. 4, p. 478, Apr. 2023, doi: 10.3390/bioengineering10040478.

SEIDEL, Stefan, Rüdiger MASCHKE, Fruhar MOZAFFARI, Regine EIBL-SCHINDLER und Dieter EIBL, 2023. Improvement of HEK293 cell growth by adapting hydrodynamic stress and predicting cell aggregate size distribution. Bioengineering. 16 April 2023. Bd. 10, Nr. 4, S. 478. DOI 10.3390/bioengineering10040478

Seidel, Stefan, Rüdiger Maschke, Fruhar Mozaffari, Regine Eibl-Schindler, and Dieter Eibl. 2023. “Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution.” Bioengineering 10 (4): 478. https://doi.org/10.3390/bioengineering10040478.

Seidel, Stefan, et al. “Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution.” Bioengineering, vol. 10, no. 4, Apr. 2023, p. 478, https://doi.org/10.3390/bioengineering10040478.