Assessment of transcriptional reprogramming of lettuce roots in response to chitin soil amendment

Li, Leilei; Kaufmann, Moritz; Makechemu, Moffat; Van Poucke, Christof; De Keyser, Ellen; Uyttendaele, Mieke; Zipfel, Cyril; Cottyn, Bart; Pothier, Joël F.

doi:10.3389/fpls.2023.1158068

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-27654

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Assessment of transcriptional reprogramming of lettuce roots in response to chitin soil amendment
Authors:	Li, Leilei Kaufmann, Moritz Makechemu, Moffat Van Poucke, Christof De Keyser, Ellen Uyttendaele, Mieke Zipfel, Cyril Cottyn, Bart Pothier, Joël F.
et. al:	No
DOI:	10.3389/fpls.2023.1158068 10.21256/zhaw-27654
Published in:	Frontiers in Plant Science
Volume(Issue):	14
Issue:	1158068
Issue Date:	5-Apr-2023
Publisher / Ed. Institution:	Frontiers Research Foundation
ISSN:	1664-462X
Language:	English
Subjects:	Chitin; Lettuce; RNA-Seq; Phenolic compound; Plant defense
Subject (DDC):	572: Biochemistry
Abstract:	Chitin soil amendment is known to improve soil quality, plant growth and stress resilience, but the underlying mechanisms are not well understood. In this study, we monitored chitin’s effect on lettuce physiology every two weeks through an eight-week growth period, analyzed the early transcriptional reprogramming and related metabolomic changes of lettuce, in response to crab chitin treatment in peat-based potting soil. In commercial growth conditions, chitin amendment still promoted lettuce growth, increased chlorophyll content, the number of leaves and crop head weight from week six. The flavonoid content in lettuce leaves was altered as well, showing an increase at week two but a decrease from week six. Transcriptomic analysis showed that over 300 genes in lettuce root were significantly differentially expressed after chitin soil treatment. Gene Ontology-term (GO) enrichment analysis revealed statistical overrepresentation of GO terms linked to photosynthesis, pigment metabolic process and phenylpropanoid metabolic process. Further analysis of the differentially expressed genes (DEGs) showed that the flavonoid pathway was mostly upregulated whereas the bifurcation of upstream phenylpropanoid pathway towards lignin biosynthesis was mostly downregulated. Metabolomic analysis revealed the upregulation of salicylic acid, chlorogenic acid, ferulic acid, and p-coumaric acid in chitin-treated lettuce seedlings. These phenolic compounds (PCs) mainly influence the phenylpropanoid biosynthesis pathway and may play important roles in plant defense reactions. Our results suggest that chitin soil amendments might activate induced resistance by priming lettuce plants and promote lettuce growth via transcriptional changes.
URI:	https://digitalcollection.zhaw.ch/handle/11475/27654
Related research data:	https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE224725
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project:	ChitinOMix – A multidisciplinary project to understand the effect of chitin soil amendment on the plant response, natural microbial community and the fate of human pathogenic bacteria
Appears in collections:	Publikationen Life Sciences und Facility Management

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Li, L., Kaufmann, M., Makechemu, M., Van Poucke, C., De Keyser, E., Uyttendaele, M., Zipfel, C., Cottyn, B., & Pothier, J. F. (2023). Assessment of transcriptional reprogramming of lettuce roots in response to chitin soil amendment. Frontiers in Plant Science, 14(1158068). https://doi.org/10.3389/fpls.2023.1158068

Li, L. et al. (2023) ‘Assessment of transcriptional reprogramming of lettuce roots in response to chitin soil amendment’, Frontiers in Plant Science, 14(1158068). Available at: https://doi.org/10.3389/fpls.2023.1158068.

L. Li et al., “Assessment of transcriptional reprogramming of lettuce roots in response to chitin soil amendment,” Frontiers in Plant Science, vol. 14, no. 1158068, Apr. 2023, doi: 10.3389/fpls.2023.1158068.

LI, Leilei, Moritz KAUFMANN, Moffat MAKECHEMU, Christof VAN POUCKE, Ellen DE KEYSER, Mieke UYTTENDAELE, Cyril ZIPFEL, Bart COTTYN und Joël F. POTHIER, 2023. Assessment of transcriptional reprogramming of lettuce roots in response to chitin soil amendment. Frontiers in Plant Science. 5 April 2023. Bd. 14, Nr. 1158068. DOI 10.3389/fpls.2023.1158068

Li, Leilei, Moritz Kaufmann, Moffat Makechemu, Christof Van Poucke, Ellen De Keyser, Mieke Uyttendaele, Cyril Zipfel, Bart Cottyn, and Joël F. Pothier. 2023. “Assessment of Transcriptional Reprogramming of Lettuce Roots in Response to Chitin Soil Amendment.” Frontiers in Plant Science 14 (1158068). https://doi.org/10.3389/fpls.2023.1158068.

Li, Leilei, et al. “Assessment of Transcriptional Reprogramming of Lettuce Roots in Response to Chitin Soil Amendment.” Frontiers in Plant Science, vol. 14, no. 1158068, Apr. 2023, https://doi.org/10.3389/fpls.2023.1158068.