The effects of heat stress on the transcriptome of human cancer cells : a meta-analysis

Scutigliani, Enzo M.; Lobo-Cerna, Fernando; Mingo Barba, Sergio; Scheidegger, Stephan; Krawczyk, Przemek M.

doi:10.3390/cancers15010113

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26892

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	The effects of heat stress on the transcriptome of human cancer cells : a meta-analysis
Authors:	Scutigliani, Enzo M. Lobo-Cerna, Fernando Mingo Barba, Sergio Scheidegger, Stephan Krawczyk, Przemek M.
et. al:	No
DOI:	10.3390/cancers15010113 10.21256/zhaw-26892
Published in:	Cancers
Volume(Issue):	15
Issue:	1
Page(s):	113
Issue Date:	24-Dec-2022
Publisher / Ed. Institution:	MDPI
ISSN:	2072-6694
Language:	English
Subjects:	Gene expression; Heat shock response; Hyperthermia; Meta-analysis; Neoplasm; Transcriptome
Subject (DDC):	616: Internal medicine and diseases
Abstract:	Hyperthermia is clinically applied cancer treatment in conjunction with radio- and/or chemotherapy, in which the tumor volume is exposed to supraphysiological temperatures. Since cells can effectively counteract the effects of hyperthermia by protective measures that are commonly known as the heat stress response, the identification of cellular processes that are essential for surviving hyperthermia could lead to novel treatment strategies that improve its therapeutic effects. Here, we apply a meta-analytic approach to 18 datasets that capture hyperthermia-induced transcriptome alterations in nine different human cancer cell lines. We find, in line with previous reports, that hyperthermia affects multiple processes, including protein folding, cell cycle, mitosis, and cell death, and additionally uncover expression changes of genes involved in KRAS signaling, inflammatory responses, TNF-a signaling and epithelial-to-mesenchymal transition (EMT). Interestingly, however, we also find a considerable inter-study variability, and an apparent absence of a 'universal' heat stress response signature, which is likely caused by the differences in experimental conditions. Our results suggest that gene expression alterations after heat stress are driven, to a large extent, by the experimental context, and call for a more extensive, controlled study that examines the effects of key experimental parameters on global gene expression patterns.
URI:	https://digitalcollection.zhaw.ch/handle/11475/26892
Related research data:	https://create.figlinq.com/dashboard/e.m.scutigliani:1055/ https://github.com/Krawczyk-Group/Scutigliani-et-al-2022
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	School of Engineering
Organisational Unit:	Institute of Applied Mathematics and Physics (IAMP)
Published as part of the ZHAW project:	Hyperboost
Appears in collections:	Publikationen School of Engineering

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Scutigliani, E. M., Lobo-Cerna, F., Mingo Barba, S., Scheidegger, S., & Krawczyk, P. M. (2022). The effects of heat stress on the transcriptome of human cancer cells : a meta-analysis. Cancers, 15(1), 113. https://doi.org/10.3390/cancers15010113

Scutigliani, E.M. et al. (2022) ‘The effects of heat stress on the transcriptome of human cancer cells : a meta-analysis’, Cancers, 15(1), p. 113. Available at: https://doi.org/10.3390/cancers15010113.

E. M. Scutigliani, F. Lobo-Cerna, S. Mingo Barba, S. Scheidegger, and P. M. Krawczyk, “The effects of heat stress on the transcriptome of human cancer cells : a meta-analysis,” Cancers, vol. 15, no. 1, p. 113, Dec. 2022, doi: 10.3390/cancers15010113.

SCUTIGLIANI, Enzo M., Fernando LOBO-CERNA, Sergio MINGO BARBA, Stephan SCHEIDEGGER und Przemek M. KRAWCZYK, 2022. The effects of heat stress on the transcriptome of human cancer cells : a meta-analysis. Cancers. 24 Dezember 2022. Bd. 15, Nr. 1, S. 113. DOI 10.3390/cancers15010113

Scutigliani, Enzo M., Fernando Lobo-Cerna, Sergio Mingo Barba, Stephan Scheidegger, and Przemek M. Krawczyk. 2022. “The Effects of Heat Stress on the Transcriptome of Human Cancer Cells : A Meta-Analysis.” Cancers 15 (1): 113. https://doi.org/10.3390/cancers15010113.

Scutigliani, Enzo M., et al. “The Effects of Heat Stress on the Transcriptome of Human Cancer Cells : A Meta-Analysis.” Cancers, vol. 15, no. 1, Dec. 2022, p. 113, https://doi.org/10.3390/cancers15010113.