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|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes|
|Published in:||Chemie Ingenieur Technik|
|Publisher / Ed. Institution:||Wiley|
|Subjects:||Chinese hamster ovary cell; Monoclonal antibody; N-1 perfusion; Ultra-high cell density|
|Subject (DDC):||660.6: Biotechnology|
|Abstract:||Various approaches to process intensification are currently being investigated to ensure time and cost savings when producing biopharmaceuticals. In the present study, a one-step inoculum production was established based on an ultra-high cell density working cell bank with immunoglobulin G producing Chinese hamster ovary cells. Cryovials were used for direct inoculation of 1-L wave-mixed perfusion bioreactors. When reaching around 180 · 106 cells mL−1 in N−1 perfusion mode, low-seed and high-seed fed-batch experiments in shake flasks were inoculated, as was the case for 250-mL stirred single-use bioreactors. Additionally, proof-of-concept runs at 50-L and 200-L scale were successfully performed. The intensification approach presented allows manufacturing capacity to be increased by up to 50 %.|
|Fulltext version:||Published version|
|License (according to publishing contract):||CC BY 4.0: Attribution 4.0 International|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Chemistry and Biotechnology (ICBT)|
|Appears in collections:||Publikationen Life Sciences und Facility Management|
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|2022_Mueller-etal_Process-intensification-one‐step-inoculum-production.pdf||461.14 kB||Adobe PDF|
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Müller, J., Ott, V., Weiss, N., Neubauer, P., Eibl, D., & Eibl, R. (2022). Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes. Chemie Ingenieur Technik, 94(12), 1977–1984. https://doi.org/10.1002/cite.202200097
Müller, J. et al. (2022) ‘Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes’, Chemie Ingenieur Technik, 94(12), pp. 1977–1984. Available at: https://doi.org/10.1002/cite.202200097.
J. Müller, V. Ott, N. Weiss, P. Neubauer, D. Eibl, and R. Eibl, “Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes,” Chemie Ingenieur Technik, vol. 94, no. 12, pp. 1977–1984, Oct. 2022, doi: 10.1002/cite.202200097.
Müller, Jan, et al. “Process Intensification Using a One‐Step Inoculum Production and High‐Seeded Fed‐Batch Processes.” Chemie Ingenieur Technik, vol. 94, no. 12, Oct. 2022, pp. 1977–84, https://doi.org/10.1002/cite.202200097.
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