Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis

Später, Thomas; Assunção, Marisa; Lit, Kwok Keung; Gong, Guidong; Wang, Xiaoling; Chen, Yi-Yun; Rao, Ying; Li, Yucong; Yiu, Chi Him Kendrick; Laschke, Matthias W.; Menger, Michael D.; Wang, Dan; Tuan, Rocky S.; Khoo, Kay-Hooi; Raghunath, Michael; Guo, Junling; Blocki, Anna

doi:10.1016/j.bioactmat.2022.03.015

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26856

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis
Authors:	Später, Thomas Assunção, Marisa Lit, Kwok Keung Gong, Guidong Wang, Xiaoling Chen, Yi-Yun Rao, Ying Li, Yucong Yiu, Chi Him Kendrick Laschke, Matthias W. Menger, Michael D. Wang, Dan Tuan, Rocky S. Khoo, Kay-Hooi Raghunath, Michael Guo, Junling Blocki, Anna
et. al:	No
DOI:	10.1016/j.bioactmat.2022.03.015 10.21256/zhaw-26856
Published in:	Bioactive Materials
Issue:	17
Page(s):	526
Pages to:	541
Issue Date:	2022
Publisher / Ed. Institution:	KeAi
ISSN:	2097-1192 2452-199X
Language:	English
Subjects:	Dextran sulfate; Extracellular matrix; Mesenchymal stem cell; Poly-electrolyte-driven co-assembly; Therapeutic angiogenesis; Wound healing
Subject (DDC):	610.28: Biomedicine, biomedical engineering
Abstract:	Tissue (re)vascularization strategies face various challenges, as therapeutic cells do not survive long enough in situ, while the administration of pro-angiogenic factors is hampered by fast clearance and insufficient ability to emulate complex spatiotemporal signaling. Here, we propose to address these limitations by engineering a functional biomaterial capable of capturing and concentrating the pro-angiogenic activities of mesenchymal stem cells (MSCs). In particular, dextran sulfate, a high molecular weight sulfated glucose polymer, supplemented to MSC cultures, interacts with MSC-derived extracellular matrix (ECM) components and facilitates their co-assembly and accumulation in the pericellular space. Upon decellularization, the resulting dextran sulfate-ECM hybrid material can be processed into MIcroparticles of SOlidified Secretome (MIPSOS). The insoluble format of MIPSOS protects protein components from degradation, while facilitating their sustained release. Proteomic analysis demonstrates that MIPSOS are highly enriched in pro-angiogenic factors, resulting in an enhanced pro-angiogenic bioactivity when compared to naïve MSC-derived ECM (cECM). Consequently, intravital microscopy of full-thickness skin wounds treated with MIPSOS demonstrates accelerated revascularization and healing, far superior to the therapeutic potential of cECM. Hence, the microparticle-based solidified stem cell secretome provides a promising platform to address major limitations of current therapeutic angiogenesis approaches.
URI:	https://digitalcollection.zhaw.ch/handle/11475/26856
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Später, T., Assunção, M., Lit, K. K., Gong, G., Wang, X., Chen, Y.-Y., Rao, Y., Li, Y., Yiu, C. H. K., Laschke, M. W., Menger, M. D., Wang, D., Tuan, R. S., Khoo, K.-H., Raghunath, M., Guo, J., & Blocki, A. (2022). Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis. Bioactive Materials, 17, 526–541. https://doi.org/10.1016/j.bioactmat.2022.03.015

Später, T. et al. (2022) ‘Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis’, Bioactive Materials, (17), pp. 526–541. Available at: https://doi.org/10.1016/j.bioactmat.2022.03.015.

T. Später et al., “Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis,” Bioactive Materials, no. 17, pp. 526–541, 2022, doi: 10.1016/j.bioactmat.2022.03.015.

SPÄTER, Thomas, Marisa ASSUNÇÃO, Kwok Keung LIT, Guidong GONG, Xiaoling WANG, Yi-Yun CHEN, Ying RAO, Yucong LI, Chi Him Kendrick YIU, Matthias W. LASCHKE, Michael D. MENGER, Dan WANG, Rocky S. TUAN, Kay-Hooi KHOO, Michael RAGHUNATH, Junling GUO und Anna BLOCKI, 2022. Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis. Bioactive Materials. 2022. Nr. 17, S. 526–541. DOI 10.1016/j.bioactmat.2022.03.015

Später, Thomas, Marisa Assunção, Kwok Keung Lit, Guidong Gong, Xiaoling Wang, Yi-Yun Chen, Ying Rao, et al. 2022. “Engineering Microparticles Based on Solidified Stem Cell Secretome with an Augmented Pro-Angiogenic Factor Portfolio for Therapeutic Angiogenesis.” Bioactive Materials, no. 17: 526–41. https://doi.org/10.1016/j.bioactmat.2022.03.015.

Später, Thomas, et al. “Engineering Microparticles Based on Solidified Stem Cell Secretome with an Augmented Pro-Angiogenic Factor Portfolio for Therapeutic Angiogenesis.” Bioactive Materials, no. 17, 2022, pp. 526–41, https://doi.org/10.1016/j.bioactmat.2022.03.015.