Full metadata record
DC FieldValueLanguage
dc.contributor.authorMa, Yujie-
dc.contributor.authorRajendran, Prayanka-
dc.contributor.authorBlum, Christian-
dc.contributor.authorCesa, Yanina-
dc.contributor.authorGartmann, Nando-
dc.contributor.authorBrühwiler, Dominik-
dc.contributor.authorSubramaniam, Vinod-
dc.date.accessioned2018-02-07T15:04:40Z-
dc.date.available2018-02-07T15:04:40Z-
dc.date.issued2011-04-01-
dc.identifier.issn0021-9797de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/2676-
dc.description.abstractThe infiltration of enhanced green fluorescent protein (EGFP) into nanochannels of different diameters in mesoporous silica particles was studied in detail by fluorescence microspectroscopy at room temperature. Silica particles from the MCM-41, ASNCs and SBA-15 families possessing nanometer-sized (3-8 nm in diameter) channels, comparable to the dimensions of the infiltrated guest protein EGFP (barrel structure with dimensions of 2.4 nm × 4.2 nm), were used as hosts. We found that it is necessary to first functionalize the surfaces of the silica particles with an amino-silane for effective encapsulation of EGFP. We demonstrated successful infiltration of the protein into the nanochannels based on fluorescence microspectroscopy and loading capacity calculations, even for nanochannel diameters approaching the protein dimensions. We studied the spatial distributions of the EGFPs within the silica particles by confocal laser scanning microscopy (CLSM) and multimode microscopy. Upon infiltration, the fluorescence lifetime drops as expected for an emitter embedded in a high refractive index medium. Further, the spectral properties of EGFP are preserved, confirming the structural integrity of the infiltrated protein. This inorganic-protein host-guest system is an example of a nanobiophotonic hybrid system that may lead to composite materials with novel optical properties.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofJournal of Colloid and Interface Sciencede_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectICBCde_CH
dc.subjectMesoporesde_CH
dc.subjectProteinsde_CH
dc.subject.ddc540: Chemiede_CH
dc.titleMicrospectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannelsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.jcis.2010.12.082de_CH
zhaw.funding.euNode_CH
zhaw.issue1de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end130de_CH
zhaw.pages.start123de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume356de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
There are no files associated with this item.
Show simple item record
Ma, Y., Rajendran, P., Blum, C., Cesa, Y., Gartmann, N., Brühwiler, D., & Subramaniam, V. (2011). Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels. Journal of Colloid and Interface Science, 356(1), 123–130. https://doi.org/10.1016/j.jcis.2010.12.082
Ma, Y. et al. (2011) ‘Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels’, Journal of Colloid and Interface Science, 356(1), pp. 123–130. Available at: https://doi.org/10.1016/j.jcis.2010.12.082.
Y. Ma et al., “Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels,” Journal of Colloid and Interface Science, vol. 356, no. 1, pp. 123–130, Apr. 2011, doi: 10.1016/j.jcis.2010.12.082.
MA, Yujie, Prayanka RAJENDRAN, Christian BLUM, Yanina CESA, Nando GARTMANN, Dominik BRÜHWILER und Vinod SUBRAMANIAM, 2011. Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels. Journal of Colloid and Interface Science. 1 April 2011. Bd. 356, Nr. 1, S. 123–130. DOI 10.1016/j.jcis.2010.12.082
Ma, Yujie, Prayanka Rajendran, Christian Blum, Yanina Cesa, Nando Gartmann, Dominik Brühwiler, and Vinod Subramaniam. 2011. “Microspectroscopic Analysis of Green Fluorescent Proteins Infiltrated into Mesoporous Silica Nanochannels.” Journal of Colloid and Interface Science 356 (1): 123–30. https://doi.org/10.1016/j.jcis.2010.12.082.
Ma, Yujie, et al. “Microspectroscopic Analysis of Green Fluorescent Proteins Infiltrated into Mesoporous Silica Nanochannels.” Journal of Colloid and Interface Science, vol. 356, no. 1, Apr. 2011, pp. 123–30, https://doi.org/10.1016/j.jcis.2010.12.082.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.