Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels

Ma, Yujie; Rajendran, Prayanka; Blum, Christian; Cesa, Yanina; Gartmann, Nando; Brühwiler, Dominik; Subramaniam, Vinod

doi:10.1016/j.jcis.2010.12.082

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels
Authors:	Ma, Yujie Rajendran, Prayanka Blum, Christian Cesa, Yanina Gartmann, Nando Brühwiler, Dominik Subramaniam, Vinod
DOI:	10.1016/j.jcis.2010.12.082
Published in:	Journal of Colloid and Interface Science
Volume(Issue):	356
Issue:	1
Page(s):	123
Pages to:	130
Issue Date:	1-Apr-2011
Publisher / Ed. Institution:	Elsevier
ISSN:	0021-9797
Language:	English
Subjects:	ICBC; Mesopores; Proteins
Subject (DDC):	540: Chemistry
Abstract:	The infiltration of enhanced green fluorescent protein (EGFP) into nanochannels of different diameters in mesoporous silica particles was studied in detail by fluorescence microspectroscopy at room temperature. Silica particles from the MCM-41, ASNCs and SBA-15 families possessing nanometer-sized (3-8 nm in diameter) channels, comparable to the dimensions of the infiltrated guest protein EGFP (barrel structure with dimensions of 2.4 nm × 4.2 nm), were used as hosts. We found that it is necessary to first functionalize the surfaces of the silica particles with an amino-silane for effective encapsulation of EGFP. We demonstrated successful infiltration of the protein into the nanochannels based on fluorescence microspectroscopy and loading capacity calculations, even for nanochannel diameters approaching the protein dimensions. We studied the spatial distributions of the EGFPs within the silica particles by confocal laser scanning microscopy (CLSM) and multimode microscopy. Upon infiltration, the fluorescence lifetime drops as expected for an emitter embedded in a high refractive index medium. Further, the spectral properties of EGFP are preserved, confirming the structural integrity of the infiltrated protein. This inorganic-protein host-guest system is an example of a nanobiophotonic hybrid system that may lead to composite materials with novel optical properties.
URI:	https://digitalcollection.zhaw.ch/handle/11475/2676
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Ma, Y., Rajendran, P., Blum, C., Cesa, Y., Gartmann, N., Brühwiler, D., & Subramaniam, V. (2011). Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels. Journal of Colloid and Interface Science, 356(1), 123–130. https://doi.org/10.1016/j.jcis.2010.12.082

Ma, Y. et al. (2011) ‘Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels’, Journal of Colloid and Interface Science, 356(1), pp. 123–130. Available at: https://doi.org/10.1016/j.jcis.2010.12.082.

Y. Ma et al., “Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels,” Journal of Colloid and Interface Science, vol. 356, no. 1, pp. 123–130, Apr. 2011, doi: 10.1016/j.jcis.2010.12.082.

MA, Yujie, Prayanka RAJENDRAN, Christian BLUM, Yanina CESA, Nando GARTMANN, Dominik BRÜHWILER und Vinod SUBRAMANIAM, 2011. Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels. Journal of Colloid and Interface Science. 1 April 2011. Bd. 356, Nr. 1, S. 123–130. DOI 10.1016/j.jcis.2010.12.082

Ma, Yujie, Prayanka Rajendran, Christian Blum, Yanina Cesa, Nando Gartmann, Dominik Brühwiler, and Vinod Subramaniam. 2011. “Microspectroscopic Analysis of Green Fluorescent Proteins Infiltrated into Mesoporous Silica Nanochannels.” Journal of Colloid and Interface Science 356 (1): 123–30. https://doi.org/10.1016/j.jcis.2010.12.082.

Ma, Yujie, et al. “Microspectroscopic Analysis of Green Fluorescent Proteins Infiltrated into Mesoporous Silica Nanochannels.” Journal of Colloid and Interface Science, vol. 356, no. 1, Apr. 2011, pp. 123–30, https://doi.org/10.1016/j.jcis.2010.12.082.