Qualification of a single‐use disk stack separator for cell separation in mammalian cell‐based antibody production

Ott, Vivian; Müller, Jan; Schirmer, Cedric; Weiss, Noémi; Göhmann, Rüdiger; Biewald, Andreas; Michel, Andrej; Eibl, Dieter; Eibl-Schindler, Regine

doi:10.1002/cite.202200096

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26259

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Qualification of a single‐use disk stack separator for cell separation in mammalian cell‐based antibody production
Authors:	Ott, Vivian Müller, Jan Schirmer, Cedric Weiss, Noémi Göhmann, Rüdiger Biewald, Andreas Michel, Andrej Eibl, Dieter Eibl-Schindler, Regine
et. al:	No
DOI:	10.1002/cite.202200096 10.21256/zhaw-26259
Published in:	Chemie Ingenieur Technik
Volume(Issue):	94
Issue:	12
Page(s):	1936
Pages to:	1943
Issue Date:	2022
Publisher / Ed. Institution:	Wiley
ISSN:	0009-286X 1522-2640
Language:	English
Subjects:	Cell separation; Chinese hamster ovary cell; Clarification efficiency; Harvest, Monoclonal antibody
Subject (DDC):	660.6: Biotechnology
Abstract:	Single-use technology is now an indispensable part of biopharmaceutical production processes. This not only applies to research and development, but also to commercial processes. The manufacturers of single-use systems are working on improving them for use in intensified processes as well as on new developments. One of the bottlenecks was the availability of single-use disk stack separators whose stainless steel variants are widely used in industry. In this study, the suitability of the new single-use disk stack separator kytero 500 was investigated for the first time for cell separation in pilot-scale Chinese hamster ovary cell-based antibody production. The cells were completely separated over a wide range of separator settings with nearly no release of intracellular substances and no loss of the antibody.
URI:	https://digitalcollection.zhaw.ch/handle/11475/26259
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Published as part of the ZHAW project:	Evaluation of cell separation using the GEA kytero® Single Use Pharma Separator with disk stack technology
Appears in collections:	Publikationen Life Sciences und Facility Management

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Ott, V., Müller, J., Schirmer, C., Weiss, N., Göhmann, R., Biewald, A., Michel, A., Eibl, D., & Eibl-Schindler, R. (2022). Qualification of a single‐use disk stack separator for cell separation in mammalian cell‐based antibody production. Chemie Ingenieur Technik, 94(12), 1936–1943. https://doi.org/10.1002/cite.202200096

Ott, V. et al. (2022) ‘Qualification of a single‐use disk stack separator for cell separation in mammalian cell‐based antibody production’, Chemie Ingenieur Technik, 94(12), pp. 1936–1943. Available at: https://doi.org/10.1002/cite.202200096.

V. Ott et al., “Qualification of a single‐use disk stack separator for cell separation in mammalian cell‐based antibody production,” Chemie Ingenieur Technik, vol. 94, no. 12, pp. 1936–1943, 2022, doi: 10.1002/cite.202200096.

OTT, Vivian, Jan MÜLLER, Cedric SCHIRMER, Noémi WEISS, Rüdiger GÖHMANN, Andreas BIEWALD, Andrej MICHEL, Dieter EIBL und Regine EIBL-SCHINDLER, 2022. Qualification of a single‐use disk stack separator for cell separation in mammalian cell‐based antibody production. Chemie Ingenieur Technik. 2022. Bd. 94, Nr. 12, S. 1936–1943. DOI 10.1002/cite.202200096

Ott, Vivian, Jan Müller, Cedric Schirmer, Noémi Weiss, Rüdiger Göhmann, Andreas Biewald, Andrej Michel, Dieter Eibl, and Regine Eibl-Schindler. 2022. “Qualification of a Single‐Use Disk Stack Separator for Cell Separation in Mammalian Cell‐Based Antibody Production.” Chemie Ingenieur Technik 94 (12): 1936–43. https://doi.org/10.1002/cite.202200096.

Ott, Vivian, et al. “Qualification of a Single‐Use Disk Stack Separator for Cell Separation in Mammalian Cell‐Based Antibody Production.” Chemie Ingenieur Technik, vol. 94, no. 12, 2022, pp. 1936–43, https://doi.org/10.1002/cite.202200096.