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|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||Qualification of a single‐use disk stack separator for cell separation in mammalian cell‐based antibody production|
|Published in:||Chemie Ingenieur Technik|
|Publisher / Ed. Institution:||Wiley|
|Subjects:||Cell separation; Chinese hamster ovary cell; Clarification efficiency; Harvest, Monoclonal antibody|
|Subject (DDC):||660.6: Biotechnology|
|Abstract:||Single-use technology is now an indispensable part of biopharmaceutical production processes. This not only applies to research and development, but also to commercial processes. The manufacturers of single-use systems are working on improving them for use in intensified processes as well as on new developments. One of the bottlenecks was the availability of single-use disk stack separators whose stainless steel variants are widely used in industry. In this study, the suitability of the new single-use disk stack separator kytero 500 was investigated for the first time for cell separation in pilot-scale Chinese hamster ovary cell-based antibody production. The cells were completely separated over a wide range of separator settings with nearly no release of intracellular substances and no loss of the antibody.|
|Fulltext version:||Published version|
|License (according to publishing contract):||CC BY 4.0: Attribution 4.0 International|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Chemistry and Biotechnology (ICBT)|
|Published as part of the ZHAW project:||Evaluation of cell separation using the GEA kytero® Single Use Pharma Separator with disk stack technology|
|Appears in collections:||Publikationen Life Sciences und Facility Management|
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|2022_Ott-etal_Qualification-single‐use-disk-stack-separator-cell-separation-mammalian.pdf||2.46 MB||Adobe PDF|
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Ott, V., Müller, J., Schirmer, C., Weiss, N., Göhmann, R., Biewald, A., Michel, A., Eibl, D., & Eibl-Schindler, R. (2022). Qualification of a single‐use disk stack separator for cell separation in mammalian cell‐based antibody production. Chemie Ingenieur Technik, 94(12), 1936–1943. https://doi.org/10.1002/cite.202200096
Ott, V. et al. (2022) ‘Qualification of a single‐use disk stack separator for cell separation in mammalian cell‐based antibody production’, Chemie Ingenieur Technik, 94(12), pp. 1936–1943. Available at: https://doi.org/10.1002/cite.202200096.
V. Ott et al., “Qualification of a single‐use disk stack separator for cell separation in mammalian cell‐based antibody production,” Chemie Ingenieur Technik, vol. 94, no. 12, pp. 1936–1943, 2022, doi: 10.1002/cite.202200096.
Ott, Vivian, et al. “Qualification of a Single‐Use Disk Stack Separator for Cell Separation in Mammalian Cell‐Based Antibody Production.” Chemie Ingenieur Technik, vol. 94, no. 12, 2022, pp. 1936–43, https://doi.org/10.1002/cite.202200096.
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