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|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||Power input measurements in stirred bioreactors at laboratory scale|
|Authors:||Kaiser, Stephan C.|
|Published in:||Journal of Visualized Experiments|
|Publisher / Ed. Institution:||JoVE|
|Subjects:||Cell culture technique; Laboratory; Bioreactor|
|Subject (DDC):||660: Chemical engineering|
|Abstract:||The power input in stirred bioreactors is an important scaling-up parameter and can be measured through the torque that acts on the impeller shaft during rotation. However, the experimental determination of the power input in small-scale vessels is still challenging due to relatively high friction losses inside typically used bushings, bearings and/or shaft seals and the accuracy of commercially available torque meters. Thus, only limited data for small-scale bioreactors, in particular single-use systems, is available in the literature, making comparisons among different single-use systems and their conventional counterparts difficult. This manuscript provides a protocol on how to measure power inputs in benchtop scale bioreactors over a wide range of turbulence conditions, which can be described by the dimensionless Reynolds number (Re). The aforementioned friction losses are effectively reduced by the use of an air bearing. The procedure on how to set up, conduct and evaluate a torque-based power input measurement, with special focus on cell culture typical agitation conditions with low to moderate turbulence (100 < Re < 2·104), is described in detail. The power input of several multi-use and single-use bioreactors is provided by the dimensionless power number (also called Newton number, P0), which is determined to be in the range of P0 ≈ 0.3 and P0 ≈ 4.5 for the maximum Reynolds numbers in the different bioreactors.|
|Fulltext version:||Published version|
|License (according to publishing contract):||CC BY-NC-ND 3.0: Attribution - Non commercial - No derivatives 3.0 Unported|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Chemistry and Biotechnology (ICBT)|
|Appears in collections:||Publikationen Life Sciences und Facility Management|
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|2018_Kaiser-etal_Power-input-measurements-stirred-bioreactors-laboratory-scale.pdf||951.01 kB||Adobe PDF|
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Kaiser, S. C., Werner, S., Jossen, V., Blaschczok, K., & Eibl, D. (2018). Power input measurements in stirred bioreactors at laboratory scale. Journal of Visualized Experiments, 135, e56078. https://doi.org/10.3791/56078
Kaiser, S.C. et al. (2018) ‘Power input measurements in stirred bioreactors at laboratory scale’, Journal of Visualized Experiments, (135), p. e56078. Available at: https://doi.org/10.3791/56078.
S. C. Kaiser, S. Werner, V. Jossen, K. Blaschczok, and D. Eibl, “Power input measurements in stirred bioreactors at laboratory scale,” Journal of Visualized Experiments, no. 135, p. e56078, 2018, doi: 10.3791/56078.
Kaiser, Stephan C., et al. “Power Input Measurements in Stirred Bioreactors at Laboratory Scale.” Journal of Visualized Experiments, no. 135, 2018, p. e56078, https://doi.org/10.3791/56078.
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