A cost effective and reliable method to predict mechanical stress in single-use and standard pumps

Dittler, Ina; Dornfeld, Wolfgang; Schöb, Reto; Cocke, Jared; Rojahn, Jürgen; Kraume, Matthias; Eibl, Dieter

doi:10.3791/53052

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	A cost effective and reliable method to predict mechanical stress in single-use and standard pumps
Authors:	Dittler, Ina Dornfeld, Wolfgang Schöb, Reto Cocke, Jared Rojahn, Jürgen Kraume, Matthias Eibl, Dieter
DOI:	10.3791/53052
Published in:	Journal of Visualized Experiments
Issue Date:	5-Aug-2015
Publisher / Ed. Institution:	JoVE
ISSN:	1940-087X
Language:	English
Subject (DDC):	620: Engineering
Abstract:	Pumps are mainly used when transferring sterile culture broths in biopharmaceutical and biotechnological production processes. However, during the pumping process shear forces occur which can lead to qualitative and/or quantitative product loss. To calculate the mechanical stress with limited experimental expense, an oil-water emulsion system was used, whose suitability was demonstrated for drop size detections in bioreactors. As drop breakup of the oil-water emulsion system is a function of mechanical stress, drop sizes need to be counted over the experimental time of shear stress investigations. In previous studies, the inline endoscopy has been shown to be an accurate and reliable measurement technique for drop size detections in liquid/liquid dispersions. The aim of this protocol is to show the suitability of the inline endoscopy technique for drop size measurements in pumping processes. In order to express the drop size, the Sauter mean diameter d32 was used as the representative diameter of drops in the oil-water emulsion. The results showed low variation in the Sauter mean diameters, which were quantified by standard deviations of below 15%, indicating the reliability of the measurement technique.
URI:	https://digitalcollection.zhaw.ch/handle/11475/2343
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Appears in collections:	Publikationen Life Sciences und Facility Management

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Dittler, I., Dornfeld, W., Schöb, R., Cocke, J., Rojahn, J., Kraume, M., & Eibl, D. (2015). A cost effective and reliable method to predict mechanical stress in single-use and standard pumps. Journal of Visualized Experiments. https://doi.org/10.3791/53052

Dittler, I. et al. (2015) ‘A cost effective and reliable method to predict mechanical stress in single-use and standard pumps’, Journal of Visualized Experiments [Preprint]. Available at: https://doi.org/10.3791/53052.

I. Dittler et al., “A cost effective and reliable method to predict mechanical stress in single-use and standard pumps,” Journal of Visualized Experiments, Aug. 2015, doi: 10.3791/53052.

DITTLER, Ina, Wolfgang DORNFELD, Reto SCHÖB, Jared COCKE, Jürgen ROJAHN, Matthias KRAUME und Dieter EIBL, 2015. A cost effective and reliable method to predict mechanical stress in single-use and standard pumps. Journal of Visualized Experiments. 5 August 2015. DOI 10.3791/53052

Dittler, Ina, Wolfgang Dornfeld, Reto Schöb, Jared Cocke, Jürgen Rojahn, Matthias Kraume, and Dieter Eibl. 2015. “A Cost Effective and Reliable Method to Predict Mechanical Stress in Single-Use and Standard Pumps.” Journal of Visualized Experiments, August. https://doi.org/10.3791/53052.

Dittler, Ina, et al. “A Cost Effective and Reliable Method to Predict Mechanical Stress in Single-Use and Standard Pumps.” Journal of Visualized Experiments, Aug. 2015, https://doi.org/10.3791/53052.