Title: A cost effective and reliable method to predict mechanical stress in single-use and standard pumps
Authors : Dittler, Ina
Dornfeld, Wolfgang
Schöb, Reto
Cocke, Jared
Rojahn, Jürgen
Kraume, Matthias
Eibl, Dieter
Published in : Journal of Visualized Experiments
Issue Date: 5-Aug-2015
Language : Englisch / English
Subject (DDC) : 620: Ingenieurwesen
Abstract: Pumps are mainly used when transferring sterile culture broths in biopharmaceutical and biotechnological production processes. However, during the pumping process shear forces occur which can lead to qualitative and/or quantitative product loss. To calculate the mechanical stress with limited experimental expense, an oil-water emulsion system was used, whose suitability was demonstrated for drop size detections in bioreactors1. As drop breakup of the oil-water emulsion system is a function of mechanical stress, drop sizes need to be counted over the experimental time of shear stress investigations. In previous studies, the inline endoscopy has been shown to be an accurate and reliable measurement technique for drop size detections in liquid/liquid dispersions. The aim of this protocol is to show the suitability of the inline endoscopy technique for drop size measurements in pumping processes. In order to express the drop size, the Sauter mean diameter d32 was used as the representative diameter of drops in the oil-water emulsion. The results showed low variation in the Sauter mean diameters, which were quantified by standard deviations of below 15%, indicating the reliability of the measurement technique.
Departement: Life Sciences und Facility Management
Publication type: Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal
DOI : 10.3791/53052
ISSN: 1940-087X
URI: https://digitalcollection.zhaw.ch/handle/11475/2343
Appears in Collections:Publikationen Life Sciences und Facility Management

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