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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-22926
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dc.contributor.authorMoretti, Chiaraluce-
dc.contributor.authorRezzonico, Fabio-
dc.contributor.authorOrfei, Benedetta-
dc.contributor.authorCortese, Chiara-
dc.contributor.authorMoreno‐Pérez, Alba-
dc.contributor.authorvan den Burg, Harrold A.-
dc.contributor.authorOnofri, Andrea-
dc.contributor.authorFirrao, Giuseppe-
dc.contributor.authorRamos, Cayo-
dc.contributor.authorSmits, Theo H.M.-
dc.contributor.authorBuonaurio, Roberto-
dc.date.accessioned2021-07-30T13:41:47Z-
dc.date.available2021-07-30T13:41:47Z-
dc.date.issued2021-07-16-
dc.identifier.issn1364-3703de_CH
dc.identifier.issn1464-6722de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/22926-
dc.description.abstractThe endophytic bacterium Pantoea agglomerans DAPP-PG 734 was previously isolated from olive knots caused by infection with Pseudomonas savastanoi pv. savastanoi DAPP-PG 722. Whole-genome analysis of this P. agglomerans strain revealed the presence of a Hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS). To assess the role of the P. agglomerans T3SS in the interaction with P. savastanoi pv. savastanoi, we generated independent knockout mutants in three Hrp genes of the P. agglomerans DAPP-PG 734 T3SS (hrpJ, hrpN, and hrpY). In contrast to the wildtype control, all three mutants failed to cause a hypersensitive response when infiltrated in tobacco leaves, suggesting that P. agglomerans T3SS is functional and injects effector proteins in plant cells. In contrast to P. savastanoi pv. savastanoi DAPP-PG 722, the wildtype strain P. agglomerans DAPP-PG 734 and its Hrp T3SS mutants did not cause olive knot disease in 1-year-old olive plants. Coinoculation of P. savastanoi pv. savastanoi with P. agglomerans wildtype strains did not significantly change the knot size, while the DAPP-PG 734 hrpY mutant induced a significant decrease in knot size, which could be complemented by providing hrpY on a plasmid. By epifluorescence microscopy and confocal laser scanning microscopy, we found that the localization patterns in knots were nonoverlapping for P. savastanoi pv. savastanoi and P. agglomerans when coinoculated. Our results suggest that suppression of olive plant defences mediated by the Hrp T3SS of P. agglomerans DAPP-PG 734 positively impacts the virulence of P. savastanoi pv. savastanoi DAPP-PG 722.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofMolecular Plant Pathologyde_CH
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/de_CH
dc.subjectPantoea agglomeransde_CH
dc.subjectPseudomonas savastanoi pv. savastanoide_CH
dc.subjectPseudomonas syringae complexde_CH
dc.subjectT3SSde_CH
dc.subjectEndophytede_CH
dc.subjectHypersensitive reactionde_CH
dc.subjectMutantde_CH
dc.subjectOlive knot communityde_CH
dc.subject.ddc579: Mikrobiologiede_CH
dc.titleSynergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722de_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Umwelt und Natürliche Ressourcen (IUNR)de_CH
dc.identifier.doi10.1111/mpp.13105de_CH
dc.identifier.doi10.21256/zhaw-22926-
dc.identifier.pmid34268839de_CH
zhaw.funding.euNode_CH
zhaw.issue10de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end1225de_CH
zhaw.pages.start1209de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume22de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedUmweltgenomikde_CH
zhaw.funding.zhawGenomik und Transkriptomik von Erwinia und Pantoeade_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
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Moretti, C., Rezzonico, F., Orfei, B., Cortese, C., Moreno‐Pérez, A., van den Burg, H. A., Onofri, A., Firrao, G., Ramos, C., Smits, T. H. M., & Buonaurio, R. (2021). Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722. Molecular Plant Pathology, 22(10), 1209–1225. https://doi.org/10.1111/mpp.13105
Moretti, C. et al. (2021) ‘Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722’, Molecular Plant Pathology, 22(10), pp. 1209–1225. Available at: https://doi.org/10.1111/mpp.13105.
C. Moretti et al., “Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722,” Molecular Plant Pathology, vol. 22, no. 10, pp. 1209–1225, Jul. 2021, doi: 10.1111/mpp.13105.
MORETTI, Chiaraluce, Fabio REZZONICO, Benedetta ORFEI, Chiara CORTESE, Alba MORENO‐PÉREZ, Harrold A. VAN DEN BURG, Andrea ONOFRI, Giuseppe FIRRAO, Cayo RAMOS, Theo H.M. SMITS und Roberto BUONAURIO, 2021. Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722. Molecular Plant Pathology. 16 Juli 2021. Bd. 22, Nr. 10, S. 1209–1225. DOI 10.1111/mpp.13105
Moretti, Chiaraluce, Fabio Rezzonico, Benedetta Orfei, Chiara Cortese, Alba Moreno‐Pérez, Harrold A. van den Burg, Andrea Onofri, et al. 2021. “Synergistic Interaction between the Type III Secretion System of the Endophytic Bacterium Pantoea Agglomerans DAPP‐PG 734 and the Virulence of the Causal Agent of Olive Knot Pseudomonas Savastanoi Pv. Savastanoi DAPP‐PG 722.” Molecular Plant Pathology 22 (10): 1209–25. https://doi.org/10.1111/mpp.13105.
Moretti, Chiaraluce, et al. “Synergistic Interaction between the Type III Secretion System of the Endophytic Bacterium Pantoea Agglomerans DAPP‐PG 734 and the Virulence of the Causal Agent of Olive Knot Pseudomonas Savastanoi Pv. Savastanoi DAPP‐PG 722.” Molecular Plant Pathology, vol. 22, no. 10, July 2021, pp. 1209–25, https://doi.org/10.1111/mpp.13105.


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