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https://doi.org/10.21256/zhaw-22926| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722 |
| Autor/-in: | Moretti, Chiaraluce Rezzonico, Fabio Orfei, Benedetta Cortese, Chiara Moreno‐Pérez, Alba van den Burg, Harrold A. Onofri, Andrea Firrao, Giuseppe Ramos, Cayo Smits, Theo H.M. Buonaurio, Roberto |
| et. al: | No |
| DOI: | 10.1111/mpp.13105 10.21256/zhaw-22926 |
| Erschienen in: | Molecular Plant Pathology |
| Band(Heft): | 22 |
| Heft: | 10 |
| Seite(n): | 1209 |
| Seiten bis: | 1225 |
| Erscheinungsdatum: | 16-Jul-2021 |
| Verlag / Hrsg. Institution: | Wiley |
| ISSN: | 1364-3703 1464-6722 |
| Sprache: | Englisch |
| Schlagwörter: | Pantoea agglomerans; Pseudomonas savastanoi pv. savastanoi; Pseudomonas syringae complex; T3SS; Endophyte; Hypersensitive reaction; Mutant; Olive knot community |
| Fachgebiet (DDC): | 579: Mikrobiologie |
| Zusammenfassung: | The endophytic bacterium Pantoea agglomerans DAPP-PG 734 was previously isolated from olive knots caused by infection with Pseudomonas savastanoi pv. savastanoi DAPP-PG 722. Whole-genome analysis of this P. agglomerans strain revealed the presence of a Hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS). To assess the role of the P. agglomerans T3SS in the interaction with P. savastanoi pv. savastanoi, we generated independent knockout mutants in three Hrp genes of the P. agglomerans DAPP-PG 734 T3SS (hrpJ, hrpN, and hrpY). In contrast to the wildtype control, all three mutants failed to cause a hypersensitive response when infiltrated in tobacco leaves, suggesting that P. agglomerans T3SS is functional and injects effector proteins in plant cells. In contrast to P. savastanoi pv. savastanoi DAPP-PG 722, the wildtype strain P. agglomerans DAPP-PG 734 and its Hrp T3SS mutants did not cause olive knot disease in 1-year-old olive plants. Coinoculation of P. savastanoi pv. savastanoi with P. agglomerans wildtype strains did not significantly change the knot size, while the DAPP-PG 734 hrpY mutant induced a significant decrease in knot size, which could be complemented by providing hrpY on a plasmid. By epifluorescence microscopy and confocal laser scanning microscopy, we found that the localization patterns in knots were nonoverlapping for P. savastanoi pv. savastanoi and P. agglomerans when coinoculated. Our results suggest that suppression of olive plant defences mediated by the Hrp T3SS of P. agglomerans DAPP-PG 734 positively impacts the virulence of P. savastanoi pv. savastanoi DAPP-PG 722. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/22926 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | CC BY-NC-ND 4.0: Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Umwelt und Natürliche Ressourcen (IUNR) |
| Publiziert im Rahmen des ZHAW-Projekts: | Genomik und Transkriptomik von Erwinia und Pantoea |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2021_Moretti-etal_Synergistic-interaction-DAPP-PG-734-and-DAPP-PG-722.pdf | 2.23 MB | Adobe PDF |  Öffnen/Anzeigen |
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Moretti, C., Rezzonico, F., Orfei, B., Cortese, C., Moreno‐Pérez, A., van den Burg, H. A., Onofri, A., Firrao, G., Ramos, C., Smits, T. H. M., & Buonaurio, R. (2021). Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722. Molecular Plant Pathology, 22(10), 1209–1225. https://doi.org/10.1111/mpp.13105
Moretti, C. et al. (2021) ‘Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722’, Molecular Plant Pathology, 22(10), pp. 1209–1225. Available at: https://doi.org/10.1111/mpp.13105.
C. Moretti et al., “Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722,” Molecular Plant Pathology, vol. 22, no. 10, pp. 1209–1225, Jul. 2021, doi: 10.1111/mpp.13105.
MORETTI, Chiaraluce, Fabio REZZONICO, Benedetta ORFEI, Chiara CORTESE, Alba MORENO‐PÉREZ, Harrold A. VAN DEN BURG, Andrea ONOFRI, Giuseppe FIRRAO, Cayo RAMOS, Theo H.M. SMITS und Roberto BUONAURIO, 2021. Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722. Molecular Plant Pathology. 16 Juli 2021. Bd. 22, Nr. 10, S. 1209–1225. DOI 10.1111/mpp.13105
Moretti, Chiaraluce, Fabio Rezzonico, Benedetta Orfei, Chiara Cortese, Alba Moreno‐Pérez, Harrold A. van den Burg, Andrea Onofri, et al. 2021. “Synergistic Interaction between the Type III Secretion System of the Endophytic Bacterium Pantoea Agglomerans DAPP‐PG 734 and the Virulence of the Causal Agent of Olive Knot Pseudomonas Savastanoi Pv. Savastanoi DAPP‐PG 722.” Molecular Plant Pathology 22 (10): 1209–25. https://doi.org/10.1111/mpp.13105.
Moretti, Chiaraluce, et al. “Synergistic Interaction between the Type III Secretion System of the Endophytic Bacterium Pantoea Agglomerans DAPP‐PG 734 and the Virulence of the Causal Agent of Olive Knot Pseudomonas Savastanoi Pv. Savastanoi DAPP‐PG 722.” Molecular Plant Pathology, vol. 22, no. 10, July 2021, pp. 1209–25, https://doi.org/10.1111/mpp.13105.
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