|Title:||Cultivation of CHO suspension cells and SEAP expression in the finesse glass bioreactor|
|Authors :||Kaiser, Stephan C.|
|Published in :||BioProcess International Industry Yearbook 2015|
|Publisher / Ed. Institution :||Informa Life Sciences Group|
|Language :||Englisch / English|
|Subject (DDC) :||660: Technische Chemie|
|Abstract:||This application note describes the cultivation of Chinese hamster ovary (CHO) suspension cells in the Finesse SmartGlass vessel bioreactor with a maximum working volume of 2.0 L. Using chemically defined minimal media, cell densities of up to 7.44 × 106 cells/mL were achieved. Recombinant secreted embryonic alkaline phosphatase (SEAP) expression was induced by medium exchange and temperature shift. Maximum SEAP activities of up to 63 U/mL were reached. A novel stirred glass bioreactor suitable for cell culture applications at benchtop scale was designed by Finesse Solutions, Inc. With a maximum working volume of 2.0 L, this bioreactor is controlled by the Finesse G3Lab controller.|
|Departement:||Life Sciences und Facility Management|
|Publication type:||Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal|
|Appears in Collections:||Publikationen Life Sciences und Facility Management|
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