Title: Cultivation of CHO suspension cells and SEAP expression in the finesse glass bioreactor
Authors : Kaiser, Stephan C.
Blaschczok, Katharina
Eibl, Dieter
Published in : BioProcess International
Publisher / Ed. Institution : Informa Life Sciences
Issue Date: 18-Aug-2015
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (publication)
Language : English
Subject (DDC) : 660: Chemical engineering
Abstract: This application note describes the cultivation of Chinese hamster ovary (CHO) suspension cells in the Finesse SmartGlass vessel bioreactor with a maximum working volume of 2.0 L. Using chemically defined minimal media, cell densities of up to 7.44 × 106 cells/mL were achieved. Recombinant secreted embryonic alkaline phosphatase (SEAP) expression was induced by medium exchange and temperature shift. Maximum SEAP activities of up to 63 U/mL were reached. A novel stirred glass bioreactor suitable for cell culture applications at benchtop scale was designed by Finesse Solutions, Inc. With a maximum working volume of 2.0 L, this bioreactor is controlled by the Finesse G3Lab controller.
Departement: Life Sciences and Facility Management
Publication type: Article in scientific journal
ISSN: 1542-6319
URI: http://www.bioprocessintl.com/sponsored-content/cultivation-of-cho-suspension-cells-and-seap-expression-in-the-finesse-glass-bioreactor
Appears in Collections:Publikationen Life Sciences und Facility Management

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