Title: Investigations on mechanical stress caused to CHO suspension cells by standard and single-use pumps
Authors : Blaschczok, Katharina
Kaiser, Stephan C.
Löffelholz, Christian
Imseng, Nicole
Burkart, Jürg
Bösch, Pascal
Dornfeld, Wolfgang
Eibl-Schindler, Regine
Eibl, Dieter
Published in : Chemie Ingenieur Technik
Volume(Issue) : 85
Issue : 1-2
Pages : 144
Pages to: 152
Publisher / Ed. Institution : Wiley VCH
Publisher / Ed. Institution: Weinheim
Issue Date: Feb-2013
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 620: Engineering
Abstract: Mechanical stress caused to transfected Chinese hamster ovary (CHO) suspension cells by reusable and single-use magnetically levitated, bearingless centrifugal pumps was investigated. Cell death rates were determined for different pump speeds and compared with data from a peristaltic and a 4-piston diaphragm pump. Furthermore, the fluid distribution inside the PuraLev® 200 pump was modeled using computational fluid dynamics. The results reveal considerably lower mechanical stress to CHO cells caused by the magnetically levitated bearingless centrifugal pumps than by the peristaltic and diaphragm pump.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1002/cite.201200135
ISSN: 0009-286X
URI: https://digitalcollection.zhaw.ch/handle/11475/2242
Appears in Collections:Publikationen Life Sciences und Facility Management

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