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Publication type: Article in scientific journal
Type of review: Open peer review
Title: 3D PCL/gelatin/genipin nanofiber sponge as scaffold for regenerative medicine
Authors: Merk, Markus
Chirikian, Orlando
Adlhart, Christian
et. al: No
DOI: 10.3390/ma14082006
Published in: Materials
Volume(Issue): 14
Issue: 8
Page(s): 2006
Issue Date: 2021
Publisher / Ed. Institution: MDPI
ISSN: 1996-1944
Language: English
Subjects: Self-assembly; 3D electrospun nanofibrous scaffold; Nanofiber aerogels; Tissue engineering; Electrospun sponge; Polycaprolactone; Biodegradation
Subject (DDC): 610.28: Biomedicine, biomedical engineering
Abstract: Recent advancements in tissue engineering and material science have radically improved in vitro culturing platforms to more accurately replicate human tissue. However, the transition to clinical relevance has been slow in part due to the lack of biologically compatible/relevant materials. In the present study, we marry the commonly used two-dimensional (2D) technique of electrospinning and a self-assembly process to construct easily reproducible, highly porous, three-dimensional (3D) nanofiber scaffolds for various tissue engineering applications. Specimens from biologically relevant polymers polycaprolactone (PCL) and gelatin were chemically cross-linked using the naturally occurring cross-linker genipin. Potential cytotoxic effects of the scaffolds were analyzed by culturing human dermal fibroblasts (HDF) up to 23 days. The 3D PCL/gelatin/genipin scaffolds produced here resemble the complex nanofibrous architecture found in naturally occurring extracellular matrix (ECM) and exhibit physiologically relevant mechanical properties as well as excellent cell cytocompatibility. Samples cross-linked with 0.5% genipin demonstrated the highest metabolic activity and proliferation rates for HDF. Scanning electron microscopy (SEM) images indicated excellent cell adhesion and the characteristic morphological features of fibroblasts in all tested samples. The three-dimensional (3D) PCL/gelatin/genipin scaffolds produced here show great potential for various 3D tissue-engineering applications such as ex vivo cell culturing platforms, wound healing, or tissue replacement.
Further description: Special Issue "Organic Nanofibers : Fabrication, Properties and Applications"
Fulltext version: Published version
License (according to publishing contract): CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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