Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells
Authors: Kremer, Antje
Wussmann, Maximiliane
Herrmann, Marietta
Raghunath, Michael
Walles, Heike
et. al: No
DOI: 10.3233/CH-190559
Published in: Clinical Hemorheology and Microcirculation
Volume(Issue): 73
Issue: 2
Page(s): 317
Pages to: 328
Issue Date: 17-Dec-2019
Publisher / Ed. Institution: IOS Press
ISSN: 1386-0291
1875-8622
Language: English
Subjects: HIF-1α; Angiogenesis; Ciclopirox olamine; Endothelial cells; Mesenchymal stem cells; Prolyl hydroxylase inhibitor; Sprouting; Vascularization; Ciclopirox; Gene expression; Human; Pathologic neovascularization
Subject (DDC): 610: Medicine and health
Abstract: Background: Prolyl hydroxylase inhibitors (PHIs) are promising compounds to promote angiogenesis by stabilizing hypoxia-inducible factor-1α (HIF-1α), a master regulator of angiogenesis. Increased HIF-1α presence induces expression of proangiogenic genes such as vascular endothelial growth factor (VEGF). Objective:We investigated the pharmacological induction of hypoxia via the PHI ciclopirox olamine (CPX) as angiogenesis strategy on human dermal microvascular endothelial cell (hd-mvEC) spheroids directly and indirectly via activating human mesenchymal stem cells (hMSCs). Methods: HMSCs were isolated from bone marrow and hd-mvECs from foreskin biopsies. MSC-conditioned medium after CPX stimulation (MSC-CM CPX) was analyzed by VEGF ELISA and Proteome Profiler™ Human Angiogenesis Array. Direct stimulation with CPX and indirect stimulation via MSC-CM CPX were compared in sprouting assays of hd-mvEC spheroids. Results: Direct stimulation with CPX significantly increased sprouting of hd-mvEC spheroids. MSC-CM CPX also induced sprouting from hd-mvEC spheroids, which was mediated by angiogenic VEGF and other proangiogenic factors that had been produced by stimulated hMSCs. Conclusions: The stimulation with CPX increased the proangiogenic response of hd-mvECs and hMSCs. The direct stimulation of hd-mvECs with CPX has the potential to replace external VEGF supplementation. Thus, CPX can induce angiogenesis in ECs even in the absence of auxiliary cells demonstrating a promising proangiogenic approach.
URI: https://digitalcollection.zhaw.ch/handle/11475/22186
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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Kremer, A., Wussmann, M., Herrmann, M., Raghunath, M., & Walles, H. (2019). Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells. Clinical Hemorheology and Microcirculation, 73(2), 317–328. https://doi.org/10.3233/CH-190559
Kremer, A. et al. (2019) ‘Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells’, Clinical Hemorheology and Microcirculation, 73(2), pp. 317–328. Available at: https://doi.org/10.3233/CH-190559.
A. Kremer, M. Wussmann, M. Herrmann, M. Raghunath, and H. Walles, “Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells,” Clinical Hemorheology and Microcirculation, vol. 73, no. 2, pp. 317–328, Dec. 2019, doi: 10.3233/CH-190559.
KREMER, Antje, Maximiliane WUSSMANN, Marietta HERRMANN, Michael RAGHUNATH und Heike WALLES, 2019. Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells. Clinical Hemorheology and Microcirculation. 17 Dezember 2019. Bd. 73, Nr. 2, S. 317–328. DOI 10.3233/CH-190559
Kremer, Antje, Maximiliane Wussmann, Marietta Herrmann, Michael Raghunath, and Heike Walles. 2019. “Ciclopirox Olamine Promotes the Angiogenic Response of Endothelial Cells and Mesenchymal Stem Cells.” Clinical Hemorheology and Microcirculation 73 (2): 317–28. https://doi.org/10.3233/CH-190559.
Kremer, Antje, et al. “Ciclopirox Olamine Promotes the Angiogenic Response of Endothelial Cells and Mesenchymal Stem Cells.” Clinical Hemorheology and Microcirculation, vol. 73, no. 2, Dec. 2019, pp. 317–28, https://doi.org/10.3233/CH-190559.


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