|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells|
|Published in:||Clinical Hemorheology and Microcirculation|
|Publisher / Ed. Institution:||IOS Press|
|Subjects:||HIF-1α; Angiogenesis; Ciclopirox olamine; Endothelial cells; Mesenchymal stem cells; Prolyl hydroxylase inhibitor; Sprouting; Vascularization; Ciclopirox; Gene expression; Human; Pathologic neovascularization|
|Subject (DDC):||610: Medicine and health|
|Abstract:||Background: Prolyl hydroxylase inhibitors (PHIs) are promising compounds to promote angiogenesis by stabilizing hypoxia-inducible factor-1α (HIF-1α), a master regulator of angiogenesis. Increased HIF-1α presence induces expression of proangiogenic genes such as vascular endothelial growth factor (VEGF). Objective:We investigated the pharmacological induction of hypoxia via the PHI ciclopirox olamine (CPX) as angiogenesis strategy on human dermal microvascular endothelial cell (hd-mvEC) spheroids directly and indirectly via activating human mesenchymal stem cells (hMSCs). Methods: HMSCs were isolated from bone marrow and hd-mvECs from foreskin biopsies. MSC-conditioned medium after CPX stimulation (MSC-CM CPX) was analyzed by VEGF ELISA and Proteome Profiler™ Human Angiogenesis Array. Direct stimulation with CPX and indirect stimulation via MSC-CM CPX were compared in sprouting assays of hd-mvEC spheroids. Results: Direct stimulation with CPX significantly increased sprouting of hd-mvEC spheroids. MSC-CM CPX also induced sprouting from hd-mvEC spheroids, which was mediated by angiogenic VEGF and other proangiogenic factors that had been produced by stimulated hMSCs. Conclusions: The stimulation with CPX increased the proangiogenic response of hd-mvECs and hMSCs. The direct stimulation of hd-mvECs with CPX has the potential to replace external VEGF supplementation. Thus, CPX can induce angiogenesis in ECs even in the absence of auxiliary cells demonstrating a promising proangiogenic approach.|
|Fulltext version:||Published version|
|License (according to publishing contract):||Licence according to publishing contract|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Chemistry and Biotechnology (ICBT)|
|Appears in collections:||Publikationen Life Sciences und Facility Management|
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Kremer, A., Wussmann, M., Herrmann, M., Raghunath, M., & Walles, H. (2019). Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells. Clinical Hemorheology and Microcirculation, 73(2), 317–328. https://doi.org/10.3233/CH-190559
Kremer, A. et al. (2019) ‘Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells’, Clinical Hemorheology and Microcirculation, 73(2), pp. 317–328. Available at: https://doi.org/10.3233/CH-190559.
A. Kremer, M. Wussmann, M. Herrmann, M. Raghunath, and H. Walles, “Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells,” Clinical Hemorheology and Microcirculation, vol. 73, no. 2, pp. 317–328, Dec. 2019, doi: 10.3233/CH-190559.
Kremer, Antje, et al. “Ciclopirox Olamine Promotes the Angiogenic Response of Endothelial Cells and Mesenchymal Stem Cells.” Clinical Hemorheology and Microcirculation, vol. 73, no. 2, Dec. 2019, pp. 317–28, https://doi.org/10.3233/CH-190559.
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