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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Methyl jasmonate enhanced production of rosmarinic acid in cell cultures of Satureja khuzistanica in a bioreactor
Authors: Khojasteh, Abbas
Mirjalili, Mohammad Hossein
Palazon, Javier
Eibl-Schindler, Regine
Cusido, Rosa M.
et. al: No
DOI: 10.1002/elsc.201600064
Published in: Engineering in Life Sciences
Volume(Issue): 16
Issue: 8
Page(s): 740
Pages to: 749
Issue Date: 2016
Publisher / Ed. Institution: Wiley
ISSN: 1618-0240
Language: English
Subjects: Cyclodextrin; Methyl jasmonate; Plant cell cultures; Rosmarinic acid; Satureja khuzistanica
Subject (DDC): 660.6: Biotechnology
Abstract: The growing interest in rosmarinic acid (RA), an ester of caffeic acid and 3,4‐dihydroxyphenyl lactic acid, is due to its biological activities, which include cognitive‐enhancing effects, slowing the development of Alzheimer's disease, cancer chemoprotection, and anti‐inflammatory activity. Inspired by the challenge of meeting the growing demand for this plant secondary metabolite, we developed a biotechnological platform based on cell suspension cultures of Satureja khuzistanica. The high amounts of RA produced by this system accumulated mainly inside the cells. To further improve production, two elicitors, 100 μM methyl jasmonate (MeJA) and 40 mM cyclodextrin (CD), were tested, separately and together. MeJA increased RA productivity more than 3‐fold, the elicited cultures achieving an RA production of 3.9 g L−1 without affecting biomass productivity. CD did not have a clear effect on RA production, and under the combined treatment of MeJA + CD only a small amount of RA was released to the medium. When the cell culture was transferred from a shake flask to a wave‐mixed bioreactor, a maximum RA production of 3.1 g L−1 and biomass productivity of 18.7 g L−1 d−1 was achieved under MeJA elicitation, demonstrating the suitability of S. khuzistanica cell suspensions for the biotechnological production of this bioactive plant secondary metabolite.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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