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dc.contributor.authorTrifan, Adriana-
dc.contributor.authorSkalicka-Woźniak, Krystyna-
dc.contributor.authorGranica, Sebastian-
dc.contributor.authorCzerwińska, Monika E.-
dc.contributor.authorKruk, Aleksandra-
dc.contributor.authorMarcourt, Laurence-
dc.contributor.authorWolfender, Jean-Luc-
dc.contributor.authorWolfram, Evelyn-
dc.contributor.authorEsslinger, Nils-
dc.contributor.authorGrubelnik, Andreas-
dc.contributor.authorLuca, Simon Vlad-
dc.date.accessioned2020-10-29T13:00:15Z-
dc.date.available2020-10-29T13:00:15Z-
dc.date.issued2020-07-31-
dc.identifier.issn0378-8741de_CH
dc.identifier.issn1872-7573de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/20712-
dc.description.abstractEthnopharmacological relevance: Symphytum officinale L. (comfrey, Boraginaceae) has been traditionally used for millennia in joint distortions, myalgia, bone fractures and hematomas. However, key activity-determining constituents and molecular mechanisms underlying its use have not been completely elucidated. Aim of the study: The objective of this study was to isolate and identify the major compounds from a hydro-ethanolic root extract of S. officinale and evaluate their antioxidant potential, alongside their effect on the cytokine production of ex vivo stimulated neutrophils, thus providing scientific support for the traditional use of comfrey root. Material and methods: Four caffeic acid oligomers were isolated from comfrey roots by liquid-liquid chroma-tography, their structures being established by MS and NMR analyses. In vitro antioxidant evaluation was per-formed by DPPH and ABTS assays. The cytotoxicity of isolated compounds was established by flow cytometry. The effect on cytokine release, such as interleukin (IL)-1β, IL-8 and tumor necrosis factor alpha (TNF-α), in lipopolysaccharide (LPS)-stimulated neutrophils was determined by enzyme-linked immunosorbent assay (ELISA). Results: The main constituents found in comfrey root were represented by four caffeic acid oligomers, namely globoidnan B (1), rabdosiin (2), rosmarinic acid (3) and globoidnan A (4). Rabdosiin, globoidnans A and B were isolated for the first time from S. officinale. In the in vitro antioxidant tests, compound 2 was the most active, with EC50 values in DPPH and ABTS assays of 29.14 ±0.43 and 11.13 ±0.39, respectively. Neutrophils’ viability over the tested concentration domain of 12.5–50 μM was not altered. At 50 μM, all compounds significantly inhibited IL-1β release, with compound 3 (45.60% release vs. LPS stimulated neutrophils) being the most active, followed by compounds 1 (53.85%), 2 (69.89%) and 4 (60.68%). Conclusions: The four caffeic acid oligomers reported in S. officinale root may contribute to the overall anti- inflammatory activity for which comfrey preparations are used in traditional medicine.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofJournal of Ethnopharmacologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectCaffeic acid oligomerde_CH
dc.subjectCountercurrent chromatographyde_CH
dc.subjectCytokine release inhibitory activityde_CH
dc.subjectGloboidnan A (PubChem CID: 56841780)de_CH
dc.subjectGloboidnan B (SpectraBase CID: 6x5Eig4VgdC)de_CH
dc.subjectInterleukin 1βde_CH
dc.subjectLiquid-liquid chromatographyde_CH
dc.subjectRabdosiin (PubChem CID: 471121)de_CH
dc.subjectRosmarinic acid (PubChem CID: 5281792)de_CH
dc.subjectStimulated neutrophilsde_CH
dc.subjectSymphytum officinalede_CH
dc.subject.ddc615: Pharmakologie und Therapeutikde_CH
dc.titleSymphytum officinale L. : liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophilsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.jep.2020.113169de_CH
dc.identifier.pmid32739565de_CH
zhaw.funding.euNode_CH
zhaw.issue113169de_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume262de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedPhytopharmaziede_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
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Trifan, A., Skalicka-Woźniak, K., Granica, S., Czerwińska, M. E., Kruk, A., Marcourt, L., Wolfender, J.-L., Wolfram, E., Esslinger, N., Grubelnik, A., & Luca, S. V. (2020). Symphytum officinale L. : liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils. Journal of Ethnopharmacology, 262(113169). https://doi.org/10.1016/j.jep.2020.113169
Trifan, A. et al. (2020) ‘Symphytum officinale L. : liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils’, Journal of Ethnopharmacology, 262(113169). Available at: https://doi.org/10.1016/j.jep.2020.113169.
A. Trifan et al., “Symphytum officinale L. : liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils,” Journal of Ethnopharmacology, vol. 262, no. 113169, Jul. 2020, doi: 10.1016/j.jep.2020.113169.
TRIFAN, Adriana, Krystyna SKALICKA-WOŹNIAK, Sebastian GRANICA, Monika E. CZERWIŃSKA, Aleksandra KRUK, Laurence MARCOURT, Jean-Luc WOLFENDER, Evelyn WOLFRAM, Nils ESSLINGER, Andreas GRUBELNIK und Simon Vlad LUCA, 2020. Symphytum officinale L. : liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils. Journal of Ethnopharmacology. 31 Juli 2020. Bd. 262, Nr. 113169. DOI 10.1016/j.jep.2020.113169
Trifan, Adriana, Krystyna Skalicka-Woźniak, Sebastian Granica, Monika E. Czerwińska, Aleksandra Kruk, Laurence Marcourt, Jean-Luc Wolfender, et al. 2020. “Symphytum Officinale L. : Liquid-Liquid Chromatography Isolation of Caffeic Acid Oligomers and Evaluation of Their Influence on Pro-Inflammatory Cytokine Release in LPS-Stimulated Neutrophils.” Journal of Ethnopharmacology 262 (113169). https://doi.org/10.1016/j.jep.2020.113169.
Trifan, Adriana, et al. “Symphytum Officinale L. : Liquid-Liquid Chromatography Isolation of Caffeic Acid Oligomers and Evaluation of Their Influence on Pro-Inflammatory Cytokine Release in LPS-Stimulated Neutrophils.” Journal of Ethnopharmacology, vol. 262, no. 113169, July 2020, https://doi.org/10.1016/j.jep.2020.113169.


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