| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Symphytum officinale L. : liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils |
| Autor/-in: | Trifan, Adriana Skalicka-Woźniak, Krystyna Granica, Sebastian Czerwińska, Monika E. Kruk, Aleksandra Marcourt, Laurence Wolfender, Jean-Luc Wolfram, Evelyn Esslinger, Nils Grubelnik, Andreas Luca, Simon Vlad |
| et. al: | No |
| DOI: | 10.1016/j.jep.2020.113169 |
| Erschienen in: | Journal of Ethnopharmacology |
| Band(Heft): | 262 |
| Heft: | 113169 |
| Erscheinungsdatum: | 31-Jul-2020 |
| Verlag / Hrsg. Institution: | Elsevier |
| ISSN: | 0378-8741 1872-7573 |
| Sprache: | Englisch |
| Schlagwörter: | Caffeic acid oligomer; Countercurrent chromatography; Cytokine release inhibitory activity; Globoidnan A (PubChem CID: 56841780); Globoidnan B (SpectraBase CID: 6x5Eig4VgdC); Interleukin 1β; Liquid-liquid chromatography; Rabdosiin (PubChem CID: 471121); Rosmarinic acid (PubChem CID: 5281792); Stimulated neutrophils; Symphytum officinale |
| Fachgebiet (DDC): | 615: Pharmakologie und Therapeutik |
| Zusammenfassung: | Ethnopharmacological relevance: Symphytum officinale L. (comfrey, Boraginaceae) has been traditionally used for millennia in joint distortions, myalgia, bone fractures and hematomas. However, key activity-determining constituents and molecular mechanisms underlying its use have not been completely elucidated. Aim of the study: The objective of this study was to isolate and identify the major compounds from a hydro-ethanolic root extract of S. officinale and evaluate their antioxidant potential, alongside their effect on the cytokine production of ex vivo stimulated neutrophils, thus providing scientific support for the traditional use of comfrey root. Material and methods: Four caffeic acid oligomers were isolated from comfrey roots by liquid-liquid chroma-tography, their structures being established by MS and NMR analyses. In vitro antioxidant evaluation was per-formed by DPPH and ABTS assays. The cytotoxicity of isolated compounds was established by flow cytometry. The effect on cytokine release, such as interleukin (IL)-1β, IL-8 and tumor necrosis factor alpha (TNF-α), in lipopolysaccharide (LPS)-stimulated neutrophils was determined by enzyme-linked immunosorbent assay (ELISA). Results: The main constituents found in comfrey root were represented by four caffeic acid oligomers, namely globoidnan B (1), rabdosiin (2), rosmarinic acid (3) and globoidnan A (4). Rabdosiin, globoidnans A and B were isolated for the first time from S. officinale. In the in vitro antioxidant tests, compound 2 was the most active, with EC50 values in DPPH and ABTS assays of 29.14 ±0.43 and 11.13 ±0.39, respectively. Neutrophils’ viability over the tested concentration domain of 12.5–50 μM was not altered. At 50 μM, all compounds significantly inhibited IL-1β release, with compound 3 (45.60% release vs. LPS stimulated neutrophils) being the most active, followed by compounds 1 (53.85%), 2 (69.89%) and 4 (60.68%). Conclusions: The four caffeic acid oligomers reported in S. officinale root may contribute to the overall anti- inflammatory activity for which comfrey preparations are used in traditional medicine. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/20712 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Trifan, A., Skalicka-Woźniak, K., Granica, S., Czerwińska, M. E., Kruk, A., Marcourt, L., Wolfender, J.-L., Wolfram, E., Esslinger, N., Grubelnik, A., & Luca, S. V. (2020). Symphytum officinale L. : liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils. Journal of Ethnopharmacology, 262(113169). https://doi.org/10.1016/j.jep.2020.113169
Trifan, A. et al. (2020) ‘Symphytum officinale L. : liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils’, Journal of Ethnopharmacology, 262(113169). Available at: https://doi.org/10.1016/j.jep.2020.113169.
A. Trifan et al., “Symphytum officinale L. : liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils,” Journal of Ethnopharmacology, vol. 262, no. 113169, Jul. 2020, doi: 10.1016/j.jep.2020.113169.
TRIFAN, Adriana, Krystyna SKALICKA-WOŹNIAK, Sebastian GRANICA, Monika E. CZERWIŃSKA, Aleksandra KRUK, Laurence MARCOURT, Jean-Luc WOLFENDER, Evelyn WOLFRAM, Nils ESSLINGER, Andreas GRUBELNIK und Simon Vlad LUCA, 2020. Symphytum officinale L. : liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils. Journal of Ethnopharmacology. 31 Juli 2020. Bd. 262, Nr. 113169. DOI 10.1016/j.jep.2020.113169
Trifan, Adriana, Krystyna Skalicka-Woźniak, Sebastian Granica, Monika E. Czerwińska, Aleksandra Kruk, Laurence Marcourt, Jean-Luc Wolfender, et al. 2020. “Symphytum Officinale L. : Liquid-Liquid Chromatography Isolation of Caffeic Acid Oligomers and Evaluation of Their Influence on Pro-Inflammatory Cytokine Release in LPS-Stimulated Neutrophils.” Journal of Ethnopharmacology 262 (113169). https://doi.org/10.1016/j.jep.2020.113169.
Trifan, Adriana, et al. “Symphytum Officinale L. : Liquid-Liquid Chromatography Isolation of Caffeic Acid Oligomers and Evaluation of Their Influence on Pro-Inflammatory Cytokine Release in LPS-Stimulated Neutrophils.” Journal of Ethnopharmacology, vol. 262, no. 113169, July 2020, https://doi.org/10.1016/j.jep.2020.113169.
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