| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | High-Performance thin-layer chromatographic fingerprint method for the detection of sennosides in Cassia senna L. and Cassia angustifolia Vahl |
| Autor/-in: | Peter, Samuel Meier, Nadja Wolfram, Evelyn Meier, Beat |
| DOI: | 10.1556/1006.2017.30.4.1 |
| Erschienen in: | Journal of Planar Chromatography - Modern TLC |
| Band(Heft): | 30 |
| Heft: | 4 |
| Seite(n): | 238 |
| Seiten bis: | 244 |
| Erscheinungsdatum: | 2017 |
| Verlag / Hrsg. Institution: | Akademiai Kiado |
| ISSN: | 0933-4173 1789-0993 |
| Sprache: | Englisch |
| Schlagwörter: | Senna; High-performance thin-layer chromatography; Identity testing; Sennoside |
| Fachgebiet (DDC): | 580: Pflanzen (Botanik) 615: Pharmakologie und Therapeutik 660.6: Biotechnologie |
| Zusammenfassung: | A Ph. Eur. herbal drug monograph requires for identification testing besides macroscopic (A) and microscopic (B) evaluation a thin-layer chromatography (TLC) fingerprint, prescribed as identification testing in subsection C. The main active constituents of Cassia senna L. and Cassia angustifolia Vahl are the dianthrone glucosides (mainly sennoside A and B). Therefore, this study presents a high-performance thin-layer chromatography (HPTLC) method which uses the sennosides as characteristic constituents. The presented method results in a fast and simple fingerprint with characteristic yellow fluorescence for the sennosides due to derivatization with a potassium hydroxide solution. The use of a senna herbal drug dry extract as system suitability test (SST) allows for a cost-effective test control. The resolution of the sennosides zones is dependent on the extractives concentration. An overload of the analytes leads to tailing (u-shaped form) and broadening as well as change in the retardation factor (Rf). The presented method is a suitable fingerprint method for Ph. Eur. conform identification C. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/1969 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Peter, S., Meier, N., Wolfram, E., & Meier, B. (2017). High-Performance thin-layer chromatographic fingerprint method for the detection of sennosides in Cassia senna L. and Cassia angustifolia Vahl. Journal of Planar Chromatography - Modern TLC, 30(4), 238–244. https://doi.org/10.1556/1006.2017.30.4.1
Peter, S. et al. (2017) ‘High-Performance thin-layer chromatographic fingerprint method for the detection of sennosides in Cassia senna L. and Cassia angustifolia Vahl’, Journal of Planar Chromatography - Modern TLC, 30(4), pp. 238–244. Available at: https://doi.org/10.1556/1006.2017.30.4.1.
S. Peter, N. Meier, E. Wolfram, and B. Meier, “High-Performance thin-layer chromatographic fingerprint method for the detection of sennosides in Cassia senna L. and Cassia angustifolia Vahl,” Journal of Planar Chromatography - Modern TLC, vol. 30, no. 4, pp. 238–244, 2017, doi: 10.1556/1006.2017.30.4.1.
PETER, Samuel, Nadja MEIER, Evelyn WOLFRAM und Beat MEIER, 2017. High-Performance thin-layer chromatographic fingerprint method for the detection of sennosides in Cassia senna L. and Cassia angustifolia Vahl. Journal of Planar Chromatography - Modern TLC. 2017. Bd. 30, Nr. 4, S. 238–244. DOI 10.1556/1006.2017.30.4.1
Peter, Samuel, Nadja Meier, Evelyn Wolfram, and Beat Meier. 2017. “High-Performance Thin-Layer Chromatographic Fingerprint Method for the Detection of Sennosides in Cassia Senna L. And Cassia Angustifolia Vahl.” Journal of Planar Chromatography - Modern TLC 30 (4): 238–44. https://doi.org/10.1556/1006.2017.30.4.1.
Peter, Samuel, et al. “High-Performance Thin-Layer Chromatographic Fingerprint Method for the Detection of Sennosides in Cassia Senna L. And Cassia Angustifolia Vahl.” Journal of Planar Chromatography - Modern TLC, vol. 30, no. 4, 2017, pp. 238–44, https://doi.org/10.1556/1006.2017.30.4.1.
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