Title: High-Performance thin-layer chromatographic fingerprint method for the detection of sennosides in Cassia senna L. and Cassia angustifolia Vahl
Authors : Peter, Samuel
Meier, Nadja
Wolfram, Evelyn
Meier, Beat
Published in : Journal of Planar Chromatography
Volume(Issue) : 30
Issue : 4
Pages : 238
Pages to: 244
Publisher / Ed. Institution : Akademiai Kiado Rt.
Issue Date: 2017
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Senna; High-performance thin-layer chromatography; Identity testing; Sennoside
Subject (DDC) : 580: Plants (Botany)
615: Pharmacology and therapeutics
660.6: Biotechnology
Abstract: A Ph. Eur. herbal drug monograph requires for identification testing besides macroscopic (A) and microscopic (B) evaluation a thin-layer chromatography (TLC) fingerprint, prescribed as identification testing in subsection C. The main active constituents of Cassia senna L. and Cassia angustifolia Vahl are the dianthrone glucosides (mainly sennoside A and B). Therefore, this study presents a high-performance thin-layer chromatography (HPTLC) method which uses the sennosides as characteristic constituents. The presented method results in a fast and simple fingerprint with characteristic yellow fluorescence for the sennosides due to derivatization with a potassium hydroxide solution. The use of a senna herbal drug dry extract as system suitability test (SST) allows for a cost-effective test control. The resolution of the sennosides zones is dependent on the extractives concentration. An overload of the analytes leads to tailing (u-shaped form) and broadening as well as change in the retardation factor (Rf). The presented method is a suitable fingerprint method for Ph. Eur. conform identification C.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1556/1006.2017.30.4.1
ISSN: 0933-4173
URI: https://digitalcollection.zhaw.ch/handle/11475/1969
Appears in Collections:Publikationen Life Sciences und Facility Management

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