|Title:||High-Performance thin-layer chromatographic fingerprint method for the detection of sennosides in Cassia senna L. and Cassia angustifolia Vahl|
|Authors :||Peter, Samuel|
|Published in :||Journal of Planar Chromatography|
|Publisher / Ed. Institution :||Akademiai Kiado Rt.|
|Language :||Englisch / English|
|Subjects :||Senna; High-performance thin-layer chromatography; Identity testing; Sennoside|
|Subject (DDC) :||580: Pflanzen (Botanik)|
615: Pharmakologie und Therapeutik
|Abstract:||A Ph. Eur. herbal drug monograph requires for identification testing besides macroscopic (A) and microscopic (B) evaluation a thin-layer chromatography (TLC) fingerprint, prescribed as identification testing in subsection C. The main active constituents of Cassia senna L. and Cassia angustifolia Vahl are the dianthrone glucosides (mainly sennoside A and B). Therefore, this study presents a high-performance thin-layer chromatography (HPTLC) method which uses the sennosides as characteristic constituents. The presented method results in a fast and simple fingerprint with characteristic yellow fluorescence for the sennosides due to derivatization with a potassium hydroxide solution. The use of a senna herbal drug dry extract as system suitability test (SST) allows for a cost-effective test control. The resolution of the sennosides zones is dependent on the extractives concentration. An overload of the analytes leads to tailing (u-shaped form) and broadening as well as change in the retardation factor (Rf). The presented method is a suitable fingerprint method for Ph. Eur. conform identification C.|
|Departement:||Life Sciences und Facility Management|
|Organisational Unit:||Institut für Chemie und Biotechnologie (ICBT)|
|Publication type:||Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal|
|Appears in Collections:||Publikationen Life Sciences und Facility Management|
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