Title: HPTLC fingerprint method for the detection of sennosides in Senna dry extracts
Authors : Meier, Nadja
Meier, Beat
Peter, Samuel
Wolfram, Evelyn
Proceedings: Planta Medica International Open
Volume(Issue) : 4
Conference details: 65th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA 2017), Basel, September 3 rd to 6th 2017
Publisher / Ed. Institution : Thieme
Issue Date: 24-Oct-2017
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Abstract)
Language : English
Subject (DDC) : 615: Pharmacology and therapeutics
660.6: Biotechnology
Abstract: In 2015 a new HPTLC method was proposed as part of the revision of the herbal drug monographs in the European Pharmacopeia Ph.Eur. of senna leafs and pods [1]. However, the proposed method was unspecific for the active constituents and showed difficulties with the repeatability in several laboratories. Therefore, alternative methods for fingerprinting were tested. The focus has been based on a fingerprint showing the active compounds which are mainly sennoside A and B and this in a fast and simple method. The mobile phase used for senna folium in the pharmacopoeia of the people's republic of China in combination with potassium hydroxide reagent showed the best result in the separation and characteristic visualization of the sennosides. The sennosides show u-shaped zones which broaden with increasing application amount. The addition of acid to the mobile phase was tested but showed no improvement. The zones are under control by the applied amount. The sennosides A and D (zones [d]) are suitable as system suitability test for the method. However, sennosides D is not easily obtainable as reference substance; therefore, a senna dry extract CRS was used as cost-effective alternative. The chromatograms of senna leafs and fruits are comparable to the ones of the senna dry extracts shown. Zoom ImageZoom Image Fig. 1: Chromatograms of different senna dry extracts SST: Senna extract CRS, R: sennoside A and B, R1/4: sennoside A and B diluted to 1/4, E1 – 7: different batches ethanolic fructus dry extract, E8/9: methanolic folia dry extracts, E10 – 12: aqueous fructus dry extracts [1] EDQM. Draft for the revision of the monographs Senna leaf (0206), Senna pods, Alexandrian (0207) and Tinnevelly (0208) – PA/PH/Exp. 13A/T (15) 19 – 21 ANP. Pharmeuropa 27.3, 169 – 186 (2015).
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Conference Poster
DOI : 10.1055/s-0037-1608524
ISSN: 2509-6656
URI: https://digitalcollection.zhaw.ch/handle/11475/1965
Appears in Collections:Publikationen Life Sciences und Facility Management

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