|Title:||Evaluation of estrogenic activity of Trifolium pratense L. and Cimicifuga racemosa L. plant extracts and formulations using the planar-YES assay|
|Authors :||Bräm, Sarah|
|Proceedings:||Planta Medica International Open|
|Conference details:||65th Annual Meeting of the Society for Medicinal Plant and Natural Product Research, Basel, September 3 rd to 6th 2017|
|Publisher / Ed. Institution :||Thieme|
|License (according to publishing contract) :||Licence according to publishing contract|
|Type of review:||Peer review (Abstract)|
|Subject (DDC) :||615: Pharmacology and therapeutics |
|Abstract:||Supplementation with natural estrogens, known as phytoestrogens are desirable for the treatment of women suffering from climacteric complaints after menopause. However, in patients with hormone-dependent cancers, such as specific types of breast cancer, the intake of phytoestrogens may be contraindicated, since some compounds are claimed to trigger the formation of metastasis-initiating cancer cells . HPTLC is an analytical tool in quality control of medicinal plants extracts. Separated compounds from multicompound extracts are fixed on the solid silica phase like a compound library. By direct coupling of the Yeast Estrogen Screen (YES) on the HPTLC plate, this compound library can also be used for screening of estrogenic activity. The reporter gene YES assay encoding the human estrogen receptor alpha (ERα) was adapted for the screening of Trifolium pratensis L. and Cimcifuga racemosa L. extracts and formulations [2,3]. The method is rapid and simple with no need for special sample preparation. Excipients such as microcrystalline cellulose, carboxymethylcellulose or polyvinyl pyrrolidone did not interfere with the method. In samples of T. pratensis, pronounced fluorescent zones of Genistein and Biochanin A could be identified, indicating estrogen receptor affinity. The half maximal effective concentration (EC50) of Genistein and Biochanin A was 2.13 and 3.34 ng, respectively. Cimicifugin showed weak estrogenic activity visible as fluorescent zones at applied masses of 4 µg. However, no ERα affinity was detected in C. racemosa samples. Formononetin interacted with ERα but was not detected in C. racemosa samples or commercial products.  Virk-Baker MK, Nagy TR, Barnes S. Planta medica 2010; 76: 1132 – 1142.  McDonnell DP, Nawaz Z, O'Malley BW. Mol Cell Biol 1991; 11: 4350 – 4355.  McDonnell DP, Nawaz Z, Densmore C, Weigel NL, Pham TA, Clark JH, O'Malley BW. J Steroid Biochem Mol Biol 1991; 39: 291 – 297.|
|Departement:||Life Sciences und Facility Management|
|Organisational Unit:||Institute of Chemistry and Biotechnology (ICBT)|
|Publication type:||Conference Poster|
|Appears in Collections:||Publikationen Life Sciences und Facility Management|
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