Title: Small scale bioprocess optimization using soft sensors
Authors : Maschke, Rüdiger
John, Gernot T.
Pretzner, Barbara
Eibl, Dieter
et. al : No
Conference details: BioProcess International Europe, Vienna, Austria, 2-4 April 2019
Issue Date: 4-Apr-2019
License (according to publishing contract) : Not specified
Type of review: No review
Language : English
Subject (DDC) : 660.6: Biotechnology
Abstract: The development and optimization of cell and microbial cultures is usually done in small scale vessel like shake flasks. However, these vessels lack the sensors and hence information provided by larger bioreactors like biomass concentration, oxygen saturation and pH-values. The proposed presentation will show approaches to overcome these hurdles for mammalian (CHO), animal (e.g. insect cells Sf9) and plant cell cultures as well as microbial fermentations. The first part covers measurement improvements for shake flasks with focus on back-scattered light for biomass growth monitoring and the utilization of sensor spots for a deeper insight into the “black box” shake flasks. In the second part, soft sensors based on engineering and biological characterizations of these vessels are introduced and explained, enabling researchers to predict process values and estimate growth curves. The last part consists of the application of mechanistic and kinetic models for an improved process understanding and an enhanced culture growth prediction
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Conference Other
URI: https://lifesciences.knect365.com/bpieurope/agenda/3#cell-culture-and-upstream-process-development_small-scale-bioprocess-optimization-using-soft-sensors
https://digitalcollection.zhaw.ch/handle/11475/17443
Appears in Collections:Publikationen Life Sciences und Facility Management

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