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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-1519
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dc.contributor.authorRezzonico, Fabio-
dc.contributor.authorDuffy, Brion-
dc.contributor.authorSmits, Theo H.M.-
dc.contributor.authorPothier, Joël F.-
dc.date.accessioned2017-12-08T09:53:17Z-
dc.date.available2017-12-08T09:53:17Z-
dc.date.issued2017-11-
dc.identifier.issn2239-7264de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/1739-
dc.description.abstractRapid and reliable identification of plant pathogenic bacteria is critical for effective implementation of phytosanitary measures. The genus Erwinia includes a number of economically important plant pathogens such as fire blight agent Erwinia amylovora or Asian pear pathogen Erwinia pyrifoliae, together with closely related plant epiphytes of unknown pathogenicity or even with a potential use for biological control like Erwinia tasmaniensis or Erwinia billingiae, respectively. Current laboratory methods to achieve satisfactory identification and discrimination between species within the Erwinia genus are based on the isolation on semi-selective media, serology, specific PCR and gene locus sequencing: these approaches are complicated and time-consuming, often requiring a priori assumptions over the identity of the isolates. Here we present a streamlined approach based on whole-cell Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) based on the AXIMA mass spectrometer of Shimadzu-Biotech Corp that demonstrates the potential of this technology for quick species identification in plant diagnostics within the genus Erwinia.de_CH
dc.language.isoende_CH
dc.publisherEdizioni ETSde_CH
dc.relation.ispartofJournal of Plant Pathologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectBacterial identificationde_CH
dc.subjectMALDI-TOF MSde_CH
dc.subjectTaxonomyde_CH
dc.subjectFire blightde_CH
dc.subject.ddc570: Biologiede_CH
dc.titleErwinia species identification using matrix-assisted laser desorption ionization-time of flight mass spectrometryde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Umwelt und Natürliche Ressourcen (IUNR)de_CH
dc.identifier.doi10.4454/jpp.v99i0.3916de_CH
dc.identifier.doi10.21256/zhaw-1519-
zhaw.funding.euNode_CH
zhaw.issueSpecial issuede_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end129de_CH
zhaw.pages.start121de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume99de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedUmweltgenomikde_CH
zhaw.funding.zhawGenomik und Transkriptomik von Erwinia und Pantoeade_CH
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Rezzonico, F., Duffy, B., Smits, T. H. M., & Pothier, J. F. (2017). Erwinia species identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. Journal of Plant Pathology, 99(Special issue), 121–129. https://doi.org/10.4454/jpp.v99i0.3916
Rezzonico, F. et al. (2017) ‘Erwinia species identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry’, Journal of Plant Pathology, 99(Special issue), pp. 121–129. Available at: https://doi.org/10.4454/jpp.v99i0.3916.
F. Rezzonico, B. Duffy, T. H. M. Smits, and J. F. Pothier, “Erwinia species identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry,” Journal of Plant Pathology, vol. 99, no. Special issue, pp. 121–129, Nov. 2017, doi: 10.4454/jpp.v99i0.3916.
REZZONICO, Fabio, Brion DUFFY, Theo H.M. SMITS und Joël F. POTHIER, 2017. Erwinia species identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. Journal of Plant Pathology. November 2017. Bd. 99, Nr. Special issue, S. 121–129. DOI 10.4454/jpp.v99i0.3916
Rezzonico, Fabio, Brion Duffy, Theo H.M. Smits, and Joël F. Pothier. 2017. “Erwinia Species Identification Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.” Journal of Plant Pathology 99 (Special issue): 121–29. https://doi.org/10.4454/jpp.v99i0.3916.
Rezzonico, Fabio, et al. “Erwinia Species Identification Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.” Journal of Plant Pathology, vol. 99, no. Special issue, Nov. 2017, pp. 121–29, https://doi.org/10.4454/jpp.v99i0.3916.


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