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https://doi.org/10.21256/zhaw-1519| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Erwinia species identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry |
| Authors: | Rezzonico, Fabio Duffy, Brion Smits, Theo H.M. Pothier, Joël F. |
| DOI: | 10.4454/jpp.v99i0.3916 10.21256/zhaw-1519 |
| Published in: | Journal of Plant Pathology |
| Volume(Issue): | 99 |
| Issue: | Special issue |
| Page(s): | 121 |
| Pages to: | 129 |
| Issue Date: | Nov-2017 |
| Publisher / Ed. Institution: | Edizioni ETS |
| ISSN: | 2239-7264 |
| Language: | English |
| Subjects: | Bacterial identification; MALDI-TOF MS; Taxonomy; Fire blight |
| Subject (DDC): | 570: Biology |
| Abstract: | Rapid and reliable identification of plant pathogenic bacteria is critical for effective implementation of phytosanitary measures. The genus Erwinia includes a number of economically important plant pathogens such as fire blight agent Erwinia amylovora or Asian pear pathogen Erwinia pyrifoliae, together with closely related plant epiphytes of unknown pathogenicity or even with a potential use for biological control like Erwinia tasmaniensis or Erwinia billingiae, respectively. Current laboratory methods to achieve satisfactory identification and discrimination between species within the Erwinia genus are based on the isolation on semi-selective media, serology, specific PCR and gene locus sequencing: these approaches are complicated and time-consuming, often requiring a priori assumptions over the identity of the isolates. Here we present a streamlined approach based on whole-cell Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) based on the AXIMA mass spectrometer of Shimadzu-Biotech Corp that demonstrates the potential of this technology for quick species identification in plant diagnostics within the genus Erwinia. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/1739 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Natural Resource Sciences (IUNR) |
| Published as part of the ZHAW project: | Genomik und Transkriptomik von Erwinia und Pantoea |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 59_Rezzonico_JPlantPathol2017.pdf | 751.06 kB | Adobe PDF |  View/Open |
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Rezzonico, F., Duffy, B., Smits, T. H. M., & Pothier, J. F. (2017). Erwinia species identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. Journal of Plant Pathology, 99(Special issue), 121–129. https://doi.org/10.4454/jpp.v99i0.3916
Rezzonico, F. et al. (2017) ‘Erwinia species identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry’, Journal of Plant Pathology, 99(Special issue), pp. 121–129. Available at: https://doi.org/10.4454/jpp.v99i0.3916.
F. Rezzonico, B. Duffy, T. H. M. Smits, and J. F. Pothier, “Erwinia species identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry,” Journal of Plant Pathology, vol. 99, no. Special issue, pp. 121–129, Nov. 2017, doi: 10.4454/jpp.v99i0.3916.
REZZONICO, Fabio, Brion DUFFY, Theo H.M. SMITS und Joël F. POTHIER, 2017. Erwinia species identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. Journal of Plant Pathology. November 2017. Bd. 99, Nr. Special issue, S. 121–129. DOI 10.4454/jpp.v99i0.3916
Rezzonico, Fabio, Brion Duffy, Theo H.M. Smits, and Joël F. Pothier. 2017. “Erwinia Species Identification Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.” Journal of Plant Pathology 99 (Special issue): 121–29. https://doi.org/10.4454/jpp.v99i0.3916.
Rezzonico, Fabio, et al. “Erwinia Species Identification Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.” Journal of Plant Pathology, vol. 99, no. Special issue, Nov. 2017, pp. 121–29, https://doi.org/10.4454/jpp.v99i0.3916.
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