| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | From particle self-assembly to functionalized sub-micron protein patterns |
| Autor/-in: | Blättler, T M Binkert, Andreas Zimmermann, M Textor, M Vörös, Janos Reimhult, E |
| DOI: | 10.1088/0957-4484/19/7/075301 |
| Erschienen in: | Nanotechnology |
| Band(Heft): | 19 |
| Heft: | 7 |
| Erscheinungsdatum: | 20-Feb-2008 |
| Verlag / Hrsg. Institution: | IOP Publishing |
| ISSN: | 0957-4484 1361-6528 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 615: Pharmakologie und Therapeutik 620: Ingenieurwesen |
| Zusammenfassung: | Biologically relevant nanopatterns are useful platforms to address fundamental questions, for example, regarding protein-protein and cell-protein interactions. For the creation of nanopatterns, complex and expensive instrumentation is often needed. We present a simple but versatile patterning method using a combination of particle and subsequent molecular self-assembly to produce ordered structures in the micron and sub-micron range. Polystyrene particles were, in a first step, assembled via dip-coating or dried in a drying cell. Silicon wafers and glass slides coated with SiO(2) and a top layer of 11 nm of TiO(2) were used as substrates. Large hexagonally ordered particle monolayers were formed with high reproducibility. These were subsequently shrunk in a controlled manner by exposure to a O(2)/N(2) plasma and subsequently used as etching masks to transfer the particle pattern onto the substrate, creating TiO(2) features in an SiO(2) background. After removing the mask the oxide contrast was translated in three simple dip-and-rinse steps into a biochemical contrast of protein-coated features in an inert background. In short, alkane phosphates were first selectively adsorbed to the TiO(2) features. Then the SiO(2) background was backfilled using poly(L-lysine)-graft-poly(ethylene glycol) and finally streptavidin was adsorbed to the hydrophobic alkane phosphate SAMs, allowing subsequent binding and hybridization of biotinylated DNA. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/1735 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | School of Engineering |
| Enthalten in den Sammlungen: | Publikationen School of Engineering |
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Blättler, T. M., Binkert, A., Zimmermann, M., Textor, M., Vörös, J., & Reimhult, E. (2008). From particle self-assembly to functionalized sub-micron protein patterns. Nanotechnology, 19(7). https://doi.org/10.1088/0957-4484/19/7/075301
Blättler, T.M. et al. (2008) ‘From particle self-assembly to functionalized sub-micron protein patterns’, Nanotechnology, 19(7). Available at: https://doi.org/10.1088/0957-4484/19/7/075301.
T. M. Blättler, A. Binkert, M. Zimmermann, M. Textor, J. Vörös, and E. Reimhult, “From particle self-assembly to functionalized sub-micron protein patterns,” Nanotechnology, vol. 19, no. 7, Feb. 2008, doi: 10.1088/0957-4484/19/7/075301.
BLÄTTLER, T M, Andreas BINKERT, M ZIMMERMANN, M TEXTOR, Janos VÖRÖS und E REIMHULT, 2008. From particle self-assembly to functionalized sub-micron protein patterns. Nanotechnology. 20 Februar 2008. Bd. 19, Nr. 7. DOI 10.1088/0957-4484/19/7/075301
Blättler, T M, Andreas Binkert, M Zimmermann, M Textor, Janos Vörös, and E Reimhult. 2008. “From Particle Self-Assembly to Functionalized Sub-Micron Protein Patterns.” Nanotechnology 19 (7). https://doi.org/10.1088/0957-4484/19/7/075301.
Blättler, T. M., et al. “From Particle Self-Assembly to Functionalized Sub-Micron Protein Patterns.” Nanotechnology, vol. 19, no. 7, Feb. 2008, https://doi.org/10.1088/0957-4484/19/7/075301.
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