Title: From particle self-assembly to functionalized sub-micron protein patterns
Authors : Blättler, T M
Binkert, Andreas
Zimmermann, M
Textor, M
Vörös, Janos
Reimhult, E
Published in : Nanotechnology
Volume(Issue) : 19
Issue : 7
Publisher / Ed. Institution : Institute of Physics Publishing
Issue Date: 20-Feb-2008
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 615: Pharmacology and therapeutics
620: Engineering
Abstract: Biologically relevant nanopatterns are useful platforms to address fundamental questions, for example, regarding protein-protein and cell-protein interactions. For the creation of nanopatterns, complex and expensive instrumentation is often needed. We present a simple but versatile patterning method using a combination of particle and subsequent molecular self-assembly to produce ordered structures in the micron and sub-micron range. Polystyrene particles were, in a first step, assembled via dip-coating or dried in a drying cell. Silicon wafers and glass slides coated with SiO(2) and a top layer of 11 nm of TiO(2) were used as substrates. Large hexagonally ordered particle monolayers were formed with high reproducibility. These were subsequently shrunk in a controlled manner by exposure to a O(2)/N(2) plasma and subsequently used as etching masks to transfer the particle pattern onto the substrate, creating TiO(2) features in an SiO(2) background. After removing the mask the oxide contrast was translated in three simple dip-and-rinse steps into a biochemical contrast of protein-coated features in an inert background. In short, alkane phosphates were first selectively adsorbed to the TiO(2) features. Then the SiO(2) background was backfilled using poly(L-lysine)-graft-poly(ethylene glycol) and finally streptavidin was adsorbed to the hydrophobic alkane phosphate SAMs, allowing subsequent binding and hybridization of biotinylated DNA.
Departement: School of Engineering
Publication type: Article in scientific Journal
DOI : 10.1088/0957-4484/19/7/075301
ISSN: 0957-4484
URI: https://digitalcollection.zhaw.ch/handle/11475/1735
Appears in Collections:Publikationen School of Engineering

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