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dc.contributor.authorKałużna, Monika-
dc.contributor.authorWillems, Anne-
dc.contributor.authorPothier, Joël F.-
dc.contributor.authorRuinelli, Michela-
dc.contributor.authorSobiczewski, Piotr-
dc.contributor.authorPuławska, Joanna-
dc.date.accessioned2017-12-04T13:52:59Z-
dc.date.available2017-12-04T13:52:59Z-
dc.date.issued2016-09-
dc.identifier.issn0723-2020de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/1663-
dc.description.abstractEight isolates of Gram-negative fluorescent bacteria (58T, 122, 374, 791, 963, 966, 970a and 1021) were obtained from diseased tissue of cherry trees from different regions of Poland. The symptoms resembled those of bacterial canker. Based on an analysis of 16S rDNA sequences the isolates shared the highest over 99.9% similarity with Pseudomonas ficuserectae JCM 2400T and Pseudomonas congelans DSM 14939T. Phylogenetic analysis using housekeeping genes gyrB, rpoD and rpoB revealed that they form a separate cluster and confirmed their closest relation to P. syringae NCPPB 281T and P. congelans LMG 21466T. DNA–DNA hybridization between the cherry isolate 58T and the type strains of these two closely related species revealed relatedness values of 58.2% and 41.9%, respectively. This was further supported by Average Nucleotide Identity (ANIb) and Genome-to-Genome Distance (GGDC) between the whole genome sequences of strain LMG 28609T and closely related Pseudomonas species. The major cellular fatty acids are 16:0 and summed feature 3 (16:1 ω7c/15:0 iso 2OH). Phenotypic characteristics differentiated the novel isolates from other closely related species. The G + C content of the genomic DNA of strain 58T was 59%. The diversity was proved by PCR MP and BOX PCR, eliminating the possibility that they constitute a clonal population. Based on the evidence of this polyphasic taxonomic study the eight strains are considered to represent a novel species of the genus Pseudomonas for which the name Pseudomonas cerasi sp. nov. (non Griffin, 1911) is proposed. The type strain of this species is 58T (= LMG 28609T = CFBP 8305T).de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofSystematic and Applied Microbiologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectPseudomonas spde_CH
dc.subjectCherry bacterial cankerde_CH
dc.subjectDNA–DNA hybridizationde_CH
dc.subjectGenomede_CH
dc.subjectMLSAde_CH
dc.subjectMALDI-TOF MSde_CH
dc.subjectFAMEde_CH
dc.subject.ddc570: Biologiede_CH
dc.titlePseudomonas cerasi sp. nov. (non Griffin, 1911) isolated from diseased tissue of cherryde_CH
dc.typeTextde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Umwelt und Natürliche Ressourcen (IUNR)de_CH
zhaw.publisher.placeAmsterdamde_CH
dc.identifier.doi10.1016/j.syapm.2016.05.005de_CH
zhaw.funding.euNode_CH
zhaw.issue6de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end377de_CH
zhaw.pages.start370de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume39de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.id.project2135de_CH
zhaw.monitoring.oajournalNot specifiedde_CH
zhaw.webfeedUmweltgenomikde_CH
Appears in Collections:Publikationen Life Sciences und Facility Management

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