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https://doi.org/10.21256/zhaw-1649| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Ribosomal protein biomarkers provide root nodule bacterial identification by MALDI-TOF MS |
| Autor/-in: | Ziegler, Dominik Pothier, Joël F. Ardley, Julie Kouakou Fossou, Romain Pflüger, Valentin de Meyer, Sofie Vogel, Guido Tonolla, Mauro Howieson, John Reeve, Wayne Perret, Xavier |
| DOI: | 10.1007/s00253-015-6515-3 10.21256/zhaw-1649 |
| Erschienen in: | Applied Microbiology and Biotechnology |
| Band(Heft): | 99 |
| Heft: | 13 |
| Seite(n): | 5547 |
| Seiten bis: | 5562 |
| Erscheinungsdatum: | 18-Mär-2015 |
| Verlag / Hrsg. Institution: | Springer |
| Verlag / Hrsg. Institution: | Berlin |
| ISSN: | 0175-7598 1432-0614 |
| Sprache: | Englisch |
| Schlagwörter: | Bacterial fingerprints; Phylogeny; Rhizobia; Legume nodules; GEBA-RNB; Cluster analysis |
| Fachgebiet (DDC): | 570: Biologie |
| Zusammenfassung: | Accurate identification of soil bacteria that form nitrogen-fixing associations with legume crops is challenging given the phylogenetic diversity of root nodule bacteria (RNB). The labor-intensive and time-consuming 16S ribosomal RNA (rRNA) sequencing and/or multilocus sequence analysis (MLSA) of conserved genes so far remain the favored molecular tools to characterize symbiotic bacteria. With the development of mass spectrometry (MS) as an alternative method to rapidly identify bacterial isolates, we recently showed that matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) can accurately characterize RNB found inside plant nodules or grown in cultures. Here, we report on the development of a MALDI-TOF RNB-specific spectral database built on whole cell MS fingerprints of 116 strains representing the major rhizobial genera. In addition to this RNB-specific module, which was successfully tested on unknown field isolates, a subset of 13 ribosomal proteins extracted from genome data was found to be sufficient for the reliable identification of nodule isolates to rhizobial species as shown in the putatively ascribed ribosomal protein masses (PARPM) database. These results reveal that data gathered from genome sequences can be used to expand spectral libraries to aid the accurate identification of bacterial species by MALDI-TOF MS. |
| Weitere Angaben: | Erworben im Rahmen der Schweizer Nationallizenzen (http://www.nationallizenzen.ch) |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/1649 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Umwelt und Natürliche Ressourcen (IUNR) |
| Publiziert im Rahmen des ZHAW-Projekts: | MALDI-TOF MS for microorganism identification: from pattern recognition towards marker based approaches |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2015_Ziegler-etal_Ribosomal-Protein-Biomarkers_AMB.pdf | 2.4 MB | Adobe PDF |  Öffnen/Anzeigen |
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Ziegler, D., Pothier, J. F., Ardley, J., Kouakou Fossou, R., Pflüger, V., de Meyer, S., Vogel, G., Tonolla, M., Howieson, J., Reeve, W., & Perret, X. (2015). Ribosomal protein biomarkers provide root nodule bacterial identification by MALDI-TOF MS. Applied Microbiology and Biotechnology, 99(13), 5547–5562. https://doi.org/10.1007/s00253-015-6515-3
Ziegler, D. et al. (2015) ‘Ribosomal protein biomarkers provide root nodule bacterial identification by MALDI-TOF MS’, Applied Microbiology and Biotechnology, 99(13), pp. 5547–5562. Available at: https://doi.org/10.1007/s00253-015-6515-3.
D. Ziegler et al., “Ribosomal protein biomarkers provide root nodule bacterial identification by MALDI-TOF MS,” Applied Microbiology and Biotechnology, vol. 99, no. 13, pp. 5547–5562, Mar. 2015, doi: 10.1007/s00253-015-6515-3.
ZIEGLER, Dominik, Joël F. POTHIER, Julie ARDLEY, Romain KOUAKOU FOSSOU, Valentin PFLÜGER, Sofie DE MEYER, Guido VOGEL, Mauro TONOLLA, John HOWIESON, Wayne REEVE und Xavier PERRET, 2015. Ribosomal protein biomarkers provide root nodule bacterial identification by MALDI-TOF MS. Applied Microbiology and Biotechnology. 18 März 2015. Bd. 99, Nr. 13, S. 5547–5562. DOI 10.1007/s00253-015-6515-3
Ziegler, Dominik, Joël F. Pothier, Julie Ardley, Romain Kouakou Fossou, Valentin Pflüger, Sofie de Meyer, Guido Vogel, et al. 2015. “Ribosomal Protein Biomarkers Provide Root Nodule Bacterial Identification by MALDI-TOF MS.” Applied Microbiology and Biotechnology 99 (13): 5547–62. https://doi.org/10.1007/s00253-015-6515-3.
Ziegler, Dominik, et al. “Ribosomal Protein Biomarkers Provide Root Nodule Bacterial Identification by MALDI-TOF MS.” Applied Microbiology and Biotechnology, vol. 99, no. 13, Mar. 2015, pp. 5547–62, https://doi.org/10.1007/s00253-015-6515-3.
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