Title: Ribosomal protein biomarkers provide root nodule bacterial identification by MALDI-TOF MS
Authors : Ziegler, Dominik
Pothier, Joël F.
Ardley, Julie
Kouakou Fossou, Romain
Pflüger, Valentin
de Meyer, Sofie
Vogel, Guido
Tonolla, Mauro
Howieson, John
Reeve, Wayne
Perret, Xavier
Published in : Applied Microbiology and Biotechnology
Volume(Issue) : 99
Issue : 13
Pages : 5547
Pages to: 5562
Publisher / Ed. Institution : Springer
Publisher / Ed. Institution: Berlin
Issue Date: 18-Mar-2015
Language : Englisch / English
Subjects : Bacterial fingerprints; Phylogeny; Rhizobia; Legume nodules; GEBA-RNB; Cluster analysis
Subject (DDC) : 570: Biologie
Abstract: Accurate identification of soil bacteria that form nitrogen-fixing associations with legume crops is challenging given the phylogenetic diversity of root nodule bacteria (RNB). The labor-intensive and time-consuming 16S ribosomal RNA (rRNA) sequencing and/or multilocus sequence analysis (MLSA) of conserved genes so far remain the favored molecular tools to characterize symbiotic bacteria. With the development of mass spectrometry (MS) as an alternative method to rapidly identify bacterial isolates, we recently showed that matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) can accurately characterize RNB found inside plant nodules or grown in cultures. Here, we report on the development of a MALDI-TOF RNB-specific spectral database built on whole cell MS fingerprints of 116 strains representing the major rhizobial genera. In addition to this RNB-specific module, which was successfully tested on unknown field isolates, a subset of 13 ribosomal proteins extracted from genome data was found to be sufficient for the reliable identification of nodule isolates to rhizobial species as shown in the putatively ascribed ribosomal protein masses (PARPM) database. These results reveal that data gathered from genome sequences can be used to expand spectral libraries to aid the accurate identification of bacterial species by MALDI-TOF MS.
Departement: Life Sciences und Facility Management
Organisational Unit: Institut für Umwelt und Natürliche Ressourcen (IUNR)
Publication type: Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal
DOI : 10.1007/s00253-015-6515-3
ISSN: 0175-7598
URI: https://digitalcollection.zhaw.ch/handle/11475/1649
Appears in Collections:Publikationen Life Sciences und Facility Management

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