Titel: Comparative genomics-informed design of two LAMP detection assays for detection of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae and discrimination of isolates belonging to the pandemic biovar 3
Autoren: Ruinelli, Michela
Schneeberger, Pierre H.H
Ferrante, Patrizia
Bühlmann, Andreas
Scortichini, Marco
Vanneste, Joel L.
Duffy, Brion
Pothier, Joël F.
Erschienen in: Plant Pathology
Band(Heft): 66
Heft: 1
Seiten: 140
Seiten bis: 149
Verlag / Hrsg. Institution: Wiley-Blackwell Publishing Ltd.
Erscheinungsdatum: 6-Jun-2016
Sprache: Englisch / English
Schlagwörter: Field based detection; Actinidiae
Fachgebiet (DDC): 570: Biologie
Zusammenfassung: The aim of this study was to develop a rapid, sensitive and reliable field-based assay for detection of the quarantine pathogen Pseudomonas syringae pv. actinidiae, the causal agent of the most destructive and economically important bacterial disease of kiwifruit. A comparative genomic approach was used on the publicly available P. syringae pv. actinidiae genomic data to select unique target regions for the development of two loop-mediated isothermal amplification (LAMP) assays able to detect P. syringae pv. actinidiae and to discriminate strains belonging to the highly virulent and globally spreading P. syringae pv. actinidiae biovar 3. Both LAMP assays showed specificity in accordance to their target and were able to detect reliably 125 CFU per reaction in less than 30 min. The developed assays were able to detect the presence of P. syringae pv. actinidiae in symptomatic as well as in asymptomatic naturally infected kiwifruit material, thus increasing the potential of the LAMP assays for phytosanitary purposes.
Departement: Life Sciences und Facility Management
Organisationseinheit: Institut für Umwelt und Natürliche Ressourcen (IUNR)
Publikationstyp: Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal
DOI: 10.1111/ppa.12551
ISSN: 0032-0862
URI: https://digitalcollection.zhaw.ch/handle/11475/1640
Enthalten in den Sammlungen:Publikationen Life Sciences und Facility Management

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