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dc.contributor.authorRuinelli, Michela-
dc.contributor.authorSchneeberger, Pierre H.H-
dc.contributor.authorFerrante, Patrizia-
dc.contributor.authorBühlmann, Andreas-
dc.contributor.authorScortichini, Marco-
dc.contributor.authorVanneste, Joel L.-
dc.contributor.authorDuffy, Brion-
dc.contributor.authorPothier, Joël F.-
dc.date.accessioned2017-11-30T15:11:27Z-
dc.date.available2017-11-30T15:11:27Z-
dc.date.issued2016-06-06-
dc.identifier.issn0032-0862de_CH
dc.identifier.issn1365-3059de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/1640-
dc.description.abstractThe aim of this study was to develop a rapid, sensitive and reliable field-based assay for detection of the quarantine pathogen Pseudomonas syringae pv. actinidiae, the causal agent of the most destructive and economically important bacterial disease of kiwifruit. A comparative genomic approach was used on the publicly available P. syringae pv. actinidiae genomic data to select unique target regions for the development of two loop-mediated isothermal amplification (LAMP) assays able to detect P. syringae pv. actinidiae and to discriminate strains belonging to the highly virulent and globally spreading P. syringae pv. actinidiae biovar 3. Both LAMP assays showed specificity in accordance to their target and were able to detect reliably 125 CFU per reaction in less than 30 min. The developed assays were able to detect the presence of P. syringae pv. actinidiae in symptomatic as well as in asymptomatic naturally infected kiwifruit material, thus increasing the potential of the LAMP assays for phytosanitary purposes.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofPlant Pathologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectField based detectionde_CH
dc.subjectActinidiaede_CH
dc.subject.ddc570: Biologiede_CH
dc.titleComparative genomics-informed design of two LAMP detection assays for detection of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae and discrimination of isolates belonging to the pandemic biovar 3de_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Umwelt und Natürliche Ressourcen (IUNR)de_CH
dc.identifier.doi10.1111/ppa.12551de_CH
zhaw.funding.euNode_CH
zhaw.issue1de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end149de_CH
zhaw.pages.start140de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume66de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.funding.snf613678de_CH
zhaw.webfeedUmweltgenomikde_CH
zhaw.funding.zhawDROPSA: Strategies to develop effective, innovative and practical approaches to protect major European fruit crops from pests and pathogensde_CH
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Ruinelli, M., Schneeberger, P. H. H., Ferrante, P., Bühlmann, A., Scortichini, M., Vanneste, J. L., Duffy, B., & Pothier, J. F. (2016). Comparative genomics-informed design of two LAMP detection assays for detection of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae and discrimination of isolates belonging to the pandemic biovar 3. Plant Pathology, 66(1), 140–149. https://doi.org/10.1111/ppa.12551
Ruinelli, M. et al. (2016) ‘Comparative genomics-informed design of two LAMP detection assays for detection of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae and discrimination of isolates belonging to the pandemic biovar 3’, Plant Pathology, 66(1), pp. 140–149. Available at: https://doi.org/10.1111/ppa.12551.
M. Ruinelli et al., “Comparative genomics-informed design of two LAMP detection assays for detection of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae and discrimination of isolates belonging to the pandemic biovar 3,” Plant Pathology, vol. 66, no. 1, pp. 140–149, Jun. 2016, doi: 10.1111/ppa.12551.
RUINELLI, Michela, Pierre H.H SCHNEEBERGER, Patrizia FERRANTE, Andreas BÜHLMANN, Marco SCORTICHINI, Joel L. VANNESTE, Brion DUFFY und Joël F. POTHIER, 2016. Comparative genomics-informed design of two LAMP detection assays for detection of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae and discrimination of isolates belonging to the pandemic biovar 3. Plant Pathology. 6 Juni 2016. Bd. 66, Nr. 1, S. 140–149. DOI 10.1111/ppa.12551
Ruinelli, Michela, Pierre H.H Schneeberger, Patrizia Ferrante, Andreas Bühlmann, Marco Scortichini, Joel L. Vanneste, Brion Duffy, and Joël F. Pothier. 2016. “Comparative Genomics-Informed Design of Two LAMP Detection Assays for Detection of the Kiwifruit Pathogen Pseudomonas Syringae Pv. Actinidiae and Discrimination of Isolates Belonging to the Pandemic Biovar 3.” Plant Pathology 66 (1): 140–49. https://doi.org/10.1111/ppa.12551.
Ruinelli, Michela, et al. “Comparative Genomics-Informed Design of Two LAMP Detection Assays for Detection of the Kiwifruit Pathogen Pseudomonas Syringae Pv. Actinidiae and Discrimination of Isolates Belonging to the Pandemic Biovar 3.” Plant Pathology, vol. 66, no. 1, June 2016, pp. 140–49, https://doi.org/10.1111/ppa.12551.


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