Title: Organomimetic skin model production based on a novel bioprinting technology
Authors : Rimann, Markus
Bleisch, Matthias
Kuster, Michael
Thurner, Marc
Meier, Christoph
Bossen, Anke
Graf-Hausner, Ursula
Conference details: 3D cell culture 2012, Congress, Dechema, Zurich, 16 March 2012
Issue Date: 2012
Language : Englisch / English
Subject (DDC) : 610: Medizin, Gesundheit
Abstract: 2013 is the deadline for the ban on sale of cosmetics and ingredients tested on animals in the EU. Thus, there is an urgent demand by the cosmetic industry for standardized and customized in vitro artificial organomimetic skin models for substance testing. Todays, human skin models are manually manufactured rather simple and not reflecting the complexity of native skin. Furthermore, quality control is only possible at the end of the production process, whereas it would be desirable to control the entire process in situ to select for properly built skin models at any time point thereby reducing costs. Bioprinting is an upcoming technology in the field of tissue engineering where cell-containing scaffolds are produced in a layer by layer deposition process in order to create 3D tissue specific models. This technique allows the creation of a biological composite system by controlling the exact deposition of cells, growth factors and extracellular matrix (ECM) molecules in a spatially controlled manner. The goal of this project is the automated production of complex and innovative full-thickness skin models with in situ quality control incorporated into a bioprinting instrument. We developed a printable hydrogel that fulfills four important criteria: It is highly viscous to maintain the structure, before it is photopolymerized within seconds, it provides cell binding sites and is cell-compatible. In order to produce a dermis equivalent alternating layers of scaffold material (PEG diacrylate, methacrylated hyaluronic acid, collagen I, photoinitiator) and human dermal fibroblasts in medium were printed. After each printed scaffold layer the structure was instantaneously photopolymerized with an integrated UV LED (365 nm ± 10 nm, 5 mW/cm2, 10 s). These cell-containing scaffolds were cultivated for 14 days in medium. The cell-containing scaffolds were stable throughout the whole time period and the fibroblast remained viable determined by MTT and spread inside the scaffold. In order to provide an in situ quality control an OCT-system was implemented into the bioprinting device. It is possible to discriminate between epidermal and dermal skin layers with images obtained with the OCT-system. This finding is crucial to analyze the differentiation progress of keratinocytes in the epidermis. We printed a fibroblast-containing 3D structure. The OCT-system is suitable for in situ quality control and is incorporated into the bioprinting instrument. Next steps include keratinocyte printing on top of the dermal layer and incorporating RGD-peptides into the scaffold to generate a fully-defined skin equivalent.  Funding: CTI, Project Number: 12148.2
Departement: Life Sciences und Facility Management
Publication type: Konferenz: Sonstiges / Conference Other
URI: https://digitalcollection.zhaw.ch/handle/11475/1613
Appears in Collections:Publikationen Life Sciences und Facility Management

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