Publikationstyp: Beitrag in wissenschaftlicher Zeitschrift
Art der Begutachtung: Peer review (Publikation)
Titel: Genomics-informed design of loop-mediated isothermal amplification for detection of phytopathogenic Xanthomonas arboricola pv. pruni at the intraspecific level
Autor/-in: Bühlmann, Andreas
Pothier, Joël F.
Tomlinson, Jennifer A.
Frey, Jürg F.
Boonham, N.
Smits, Theo H.M.
Duffy, Brion
DOI: 10.1111/j.1365-3059.2012.02654.x
Erschienen in: Plant Pathology
Band(Heft): 62
Heft: 2
Seite(n): 475
Seiten bis: 484
Erscheinungsdatum: 2-Jul-2012
Verlag / Hrsg. Institution: Wiley
ISSN: 0032-0862
1365-3059
Sprache: Englisch
Fachgebiet (DDC): 570: Biologie
Zusammenfassung: The objective of this study was to develop a rapid, sensitive detection assay for the quarantine pathogen Xanthomonas arboricola pv. pruni, causal agent of stone fruit bacterial spot, an economically important disease of Prunus spp. Unique targets were identified from X. arboricola pv. pruni genomes using a comparative genomics pipeline of other Xanthomonas species, subspecies and pathovars, and used to identify specific diagnostic markers. Loop-mediated isothermal amplification (LAMP) was then applied to these markers to provide rapid, sensitive and specific detection. The method developed showed unrivalled specificity with the 79 tested strains and, in contrast to previously established techniques, distinguished between phylogenetically close subspecies such as X. arboricola pv. corylina. The sensitivity of this test is comparable to that of a previously reported TaqMan™ assay at 103 CFU mL−1, while the unrivalled speed of LAMP technology enables a positive result to be obtained in <15 min. The developed assay can be used with real-time fluorescent detectors for quantitative results as well as with DNA-staining dyes to function as a simplified strategy for on-site pathogen detection.
URI: https://digitalcollection.zhaw.ch/handle/11475/1592
Volltext Version: Publizierte Version
Lizenz (gemäss Verlagsvertrag): Lizenz gemäss Verlagsvertrag
Departement: Life Sciences und Facility Management
Organisationseinheit: Institut für Umwelt und Natürliche Ressourcen (IUNR)
Publiziert im Rahmen des ZHAW-Projekts: Comparative genomics of the plant pathogen Xanthomonas arboricola pv. pruni and related xanthomonads
Enthalten in den Sammlungen:Publikationen Life Sciences und Facility Management

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Bühlmann, A., Pothier, J. F., Tomlinson, J. A., Frey, J. F., Boonham, N., Smits, T. H. M., & Duffy, B. (2012). Genomics-informed design of loop-mediated isothermal amplification for detection of phytopathogenic Xanthomonas arboricola pv. pruni at the intraspecific level. Plant Pathology, 62(2), 475–484. https://doi.org/10.1111/j.1365-3059.2012.02654.x
Bühlmann, A. et al. (2012) ‘Genomics-informed design of loop-mediated isothermal amplification for detection of phytopathogenic Xanthomonas arboricola pv. pruni at the intraspecific level’, Plant Pathology, 62(2), pp. 475–484. Available at: https://doi.org/10.1111/j.1365-3059.2012.02654.x.
A. Bühlmann et al., “Genomics-informed design of loop-mediated isothermal amplification for detection of phytopathogenic Xanthomonas arboricola pv. pruni at the intraspecific level,” Plant Pathology, vol. 62, no. 2, pp. 475–484, Jul. 2012, doi: 10.1111/j.1365-3059.2012.02654.x.
BÜHLMANN, Andreas, Joël F. POTHIER, Jennifer A. TOMLINSON, Jürg F. FREY, N. BOONHAM, Theo H.M. SMITS und Brion DUFFY, 2012. Genomics-informed design of loop-mediated isothermal amplification for detection of phytopathogenic Xanthomonas arboricola pv. pruni at the intraspecific level. Plant Pathology. 2 Juli 2012. Bd. 62, Nr. 2, S. 475–484. DOI 10.1111/j.1365-3059.2012.02654.x
Bühlmann, Andreas, Joël F. Pothier, Jennifer A. Tomlinson, Jürg F. Frey, N. Boonham, Theo H.M. Smits, and Brion Duffy. 2012. “Genomics-Informed Design of Loop-Mediated Isothermal Amplification for Detection of Phytopathogenic Xanthomonas Arboricola Pv. Pruni at the Intraspecific Level.” Plant Pathology 62 (2): 475–84. https://doi.org/10.1111/j.1365-3059.2012.02654.x.
Bühlmann, Andreas, et al. “Genomics-Informed Design of Loop-Mediated Isothermal Amplification for Detection of Phytopathogenic Xanthomonas Arboricola Pv. Pruni at the Intraspecific Level.” Plant Pathology, vol. 62, no. 2, July 2012, pp. 475–84, https://doi.org/10.1111/j.1365-3059.2012.02654.x.


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