Title: Genomics-informed design of loop-mediated isothermal amplification for detection of phytopathogenic Xanthomonas arboricola pv. pruni at the intraspecific level
Authors : Bühlmann, Andreas
Pothier, Joël F.
Tomlinson, Jennifer A.
Frey, Jürg F.
Boonham, N.
Smits, Theo H.M.
Duffy, Brion
Published in : Plant Pathology
Volume(Issue) : 62
Issue : 2
Pages : 475
Pages to: 484
Publisher / Ed. Institution : Wiley-Blackwell Publishing Ltd.
Issue Date: 2-Jul-2012
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 570: Biology
Abstract: The objective of this study was to develop a rapid, sensitive detection assay for the quarantine pathogen Xanthomonas arboricola pv. pruni, causal agent of stone fruit bacterial spot, an economically important disease of Prunus spp. Unique targets were identified from X. arboricola pv. pruni genomes using a comparative genomics pipeline of other Xanthomonas species, subspecies and pathovars, and used to identify specific diagnostic markers. Loop-mediated isothermal amplification (LAMP) was then applied to these markers to provide rapid, sensitive and specific detection. The method developed showed unrivalled specificity with the 79 tested strains and, in contrast to previously established techniques, distinguished between phylogenetically close subspecies such as X. arboricola pv. corylina. The sensitivity of this test is comparable to that of a previously reported TaqMan™ assay at 103 CFU mL−1, while the unrivalled speed of LAMP technology enables a positive result to be obtained in <15 min. The developed assay can be used with real-time fluorescent detectors for quantitative results as well as with DNA-staining dyes to function as a simplified strategy for on-site pathogen detection.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Publication type: Article in scientific Journal
DOI : 10.1111/j.1365-3059.2012.02654.x
ISSN: 0032-0862
URI: https://digitalcollection.zhaw.ch/handle/11475/1592
Published as part of the ZHAW project : Comparative genomics of the plant pathogen Xanthomonas arboricola pv. pruni and related xanthomonads
Appears in Collections:Publikationen Life Sciences und Facility Management

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