Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-1469
Title: Comparative genomics of 12 strains of Erwinia amylovora identifies a pan-genome with a large conserved core
Authors : Mann, Rachel A.
Smits, Theo H.M.
Bühlmann, Andreas
Blom, Jochen
Goesmann, Alexander
Frey, Jürg E.
Plummer, Kim M.
Beer, Steven V.
Luck, Joanne
Duffy, Brion
Rodoni, Brendan
Published in : PLoS One
Volume(Issue) : 8
Issue : 2
Publisher / Ed. Institution : Public Library of Science
Publisher / Ed. Institution: San Francisco
Issue Date: 7-Feb-2013
Language : Englisch / English
Subject (DDC) : 570: Biologie
Abstract: The plant pathogen Erwinia amylovora can be divided into two host-specific groupings; strains infecting a broad range of hosts within the Rosaceae subfamily Spiraeoideae (e.g., Malus, Pyrus, Crataegus, Sorbus) and strains infecting Rubus (raspberries and blackberries). Comparative genomic analysis of 12 strains representing distinct populations (e.g., geographic, temporal, host origin) of E. amylovora was used to describe the pan-genome of this major pathogen. The pan-genome contains 5751 coding sequences and is highly conserved relative to other phytopathogenic bacteria comprising on average 89% conserved, core genes. The chromosomes of Spiraeoideae-infecting strains were highly homogeneous, while greater genetic diversity was observed between Spiraeoideae- and Rubus-infecting strains (and among individual Rubus-infecting strains), the majority of which was attributed to variable genomic islands. Based on genomic distance scores and phylogenetic analysis, the Rubus-infecting strain ATCC BAA-2158 was genetically more closely related to the Spiraeoideae-infecting strains of E. amylovora than it was to the other Rubus-infecting strains. Analysis of the accessory genomes of Spiraeoideae- and Rubus-infecting strains has identified putative host-specific determinants including variation in the effector protein HopX1Ea and a putative secondary metabolite pathway only present in Rubus-infecting strains.
Departement: Life Sciences und Facility Management
Organisational Unit: Institut für Umwelt und Natürliche Ressourcen (IUNR)
Publication type: Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal
DOI : 10.1371/journal.pone.0055644
10.21256/zhaw-1469
ISSN: 1932-6203
URI: https://digitalcollection.zhaw.ch/handle/11475/1591
Appears in Collections:Publikationen Life Sciences und Facility Management

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